Human PADI4 ELISA Kit | PADI4 elisa kit

Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit

Cat. Num. AAA2505910

• Gene Names: PADI4; PAD; PAD4; PDI4; PDI5; PADI5
• Reactivity: Human
• Synonyms: PADI4; Human PADI4 (Peptidyl Arginine Deiminase Type IV) ELISA Kit; PADI4 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human PADI4 in samples. No significant cross-reactivity or interference between Human PADI4 and analogues was observed.
• Sequence Length: 663

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.19-12ng/mL
• Sensitivity: 0.11ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PADI4 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PADI4 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PADI4 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human PADI4 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PADI4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PADI4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PADI4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human PADI4. You can calculate the concentration of Human PADI4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 216548487
• NCBI GeneID: 23569
• NCBI Accession #: NP_036519.2
• NCBI GenBank Nucleotide #: NM_012387.2
• UniProt Accession #: Q9UM07; Q5VTZ8; Q70SX4; A8K392; B2RBW0
• Molecular Weight: 74,079 Da
• NCBI Official Full Name: protein-arginine deiminase type-4
• NCBI Official Synonym Full Names: peptidyl arginine deiminase, type IV
• NCBI Official Symbol: PADI4
• NCBI Official Synonym Symbols: PAD; PAD4; PDI4; PDI5; PADI5
• NCBI Protein Information: protein-arginine deiminase type-4; HL-60 PAD; PADI-H protein; peptidyl arginine deiminase, type V; protein-arginine deiminase type IV
• UniProt Protein Name: Protein-arginine deiminase type-4
• Protein Family: Protein-arginine deiminase
• UniProt Gene Name: PADI4
• UniProt Synonym Gene Names: PADI5; PDI5
• UniProt Entry Name: PADI4_HUMAN

NCBI Description

This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Uniprot Description

PADI4: Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA). It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. Belongs to the protein arginine deiminase family.

Protein type: Nuclear receptor co-regulator; EC 3.5.3.15; Hydrolase

Chromosomal Location of Human Ortholog: 1p36.13

Cellular Component: cytoplasm; nucleus

Molecular Function: arginine deiminase activity; protein binding; calcium ion binding; protein-arginine deiminase activity

Biological Process: chromatin remodeling; nucleosome assembly; transcription, DNA-dependent; regulation of transcription, DNA-dependent; stem cell maintenance; protein modification process; innate immune response; chromatin modification; peptidyl-citrulline biosynthetic process from peptidyl-arginine

Disease: Rheumatoid Arthritis