Testing Data
Antibody Diluent Reagent
Antibody Diluent for Western Blotting
Applications
Western Blot
Synonyms
Antibody Diluent; Antibody Diluent for Western Blotting; Antibody Diluent reagent
Form/Format
Liquid
Applicable Applications for Antibody Diluent reagent
Western Blot (WB)
Application Notes
This diluent is used to dilute the primary or secondary antibody when performing Western Blot.
Assay Principle
Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of the primary antibody in WB and effectively increase storage time of diluted primary antibody.
Usage
Refer to the instructions for use of the primary antibody and the expression level of the target protein in the sample, and dilute the primary antibody in an appropriate ratio. Diluted primary antibody can be used directly in Western. Unused diluted primary antibody can be stored at 4 degree C for future use.
Notes
1. In order to enable the antibody to be used repeatedly, it is recommended to perform the binding reaction between the primary antibody and the protein membrane at 4 degree C to slow down the decay rate of the primary antibody.
2. Please do not use the Antibody Diluent if there is any precipitation or bacterial contamination.
2. Please do not use the Antibody Diluent if there is any precipitation or bacterial contamination.
Preparation and Storage
Store at 4 degree C for one year. Avoid multiple freeze-thaw cycles.
Testing Data
Testing Data
Related Product Information for Antibody Diluent reagent
Antibody Diluent for Western Blotting consists of BSA, detergent and antibody stabilizer. This buffer helps to reduce non-specific binding of primary antibody in WB and effectively increase storage time of the primary antibody working solution.
Product Categories/Family for Antibody Diluent reagent
Similar Products
Product Notes
The Antibody Diluent (Catalog #AAA1753880) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Antibody Diluent can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). This diluent is used to dilute the primary or secondary antibody when performing Western Blot. Researchers should empirically determine the suitability of the Antibody Diluent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Antibody Diluent, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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