1 X Trypsin-0.53mM EDTA Solution Reagent
1 X Trypsin-0.53mM EDTA Solution
Applications
Cell Assay
Synonyms
1 X Trypsin-0.53mM EDTA Solution; 1 X Trypsin-0.53mM EDTA Solution reagent
Form/Format
Frozen. Sterile filtered, pH7.0-9.0
Applicable Applications for 1 X Trypsin-0.53mM EDTA Solution reagent
Cell Assay
Biological Source
Porcine
Biochemical/Physiological Actions
Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reactionis slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is onthe carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave esterand amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing theseions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.
Components
Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminalhexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleavingat the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.
Note
This product does contain phenol red. Due to shipment on dry ice, there could be significant carbon dioxidebuildup in the package. This CO2 may enter the solution and lower the pHslightly, giving an orange rather than pinkish color. The orange solution will still be suitable for use, or the pHcan be adjusted with sodium hydroxide. Incubating cells with too high a trypsin concentration for a long period can damage cell membranes and kill the cells. Solubilizing trypsin or diluting it from a concentrated solution should be done with a buffered salt solution containingno Ca2+ or Mg2+.
Preparation and Storage
Store the solution between -10 degree C and -40 degree C. Avoid repeated freeze thaw cycles
Shipping: Shipped with dry ice
Shipping: Shipped with dry ice
Related Product Information for 1 X Trypsin-0.53mM EDTA Solution reagent
The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.
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Product Notes
The 1 X Trypsin-0.53mM EDTA Solution (Catalog #AAA556499) is a Reagent and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 1 X Trypsin-0.53mM EDTA Solution can be used in a range of immunoassay formats including, but not limited to, Cell Assay. Researchers should empirically determine the suitability of the 1 X Trypsin-0.53mM EDTA Solution for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "1 X Trypsin-0.53mM EDTA Solution, Reagent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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