Bovine Retinol Binding Protein 4 ELISA Kit | RBP4 elisa kit

Bovine Retinol Binding Protein 4 ELISA Kit

Cat. Num. AAA737578

• Gene Names: Rbp4; RBPA
• Reactivity: Bovine
• Synonyms: Retinol Binding Protein 4; Bovine Retinol Binding Protein 4 ELISA Kit; RBP4 elisa kit

• Reactivity: Bovine

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RBP4 elisa kit

Intended Uses: This RARalpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human RARalpha. This ELISA kit for research use only!

Principle of the Assay: RARalpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-RARalpha antibody and an RARalpha-HRP conjugate. The assay sample and buffer are incubated together with RARalpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RARalpha concentration since RARalpha from samples and RARalpha-HRP conjugate compete for the anti-RARalpha antibody binding site. Since the number of sites is limited, as more sites are occupied by RARalpha from the sample, fewer sites are left to bind RARalpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RARalpha concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RBP4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 158187535
• NCBI GeneID: 25703
• NCBI Accession #: NP_037294.1
• NCBI GenBank Nucleotide #: NM_013162.1
• Molecular Weight: 23,220 Da
• NCBI Official Full Name: retinol-binding protein 4
• NCBI Official Synonym Full Names: retinol binding protein 4, plasma
• NCBI Official Symbol: Rbp4
• NCBI Official Synonym Symbols: RBPA
• NCBI Protein Information: retinol-binding protein 4; RBP; PRBP; plasma retinol-binding protein
• UniProt Protein Name: Retinol-binding protein 4
• Protein Family: Retinol-binding protein
• UniProt Gene Name: Rbp4
• UniProt Synonym Gene Names: PRBP; RBP
• UniProt Entry Name: RET4_RAT

NCBI Description

may play a role in vitamin A transport [RGD, Feb 2006]

Uniprot Description

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.