Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit | WNT3 elisa kit

Rat Wingless Type MMTV Integration Site Family, Member 3 (WNT3) ELISA Kit

Cat. Num. AAA2019205

• Gene Names: WNT3; INT4; TETAMS
• Reactivity: Rat
• Synonyms: Wingless Type MMTV Integration Site Family; Member 3 (WNT3); Rat Wingless Type MMTV Integration Site Family; Member 3 (WNT3) ELISA Kit; INT4; Proto-oncogene Int-4 homolog; WNT3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3 (WNT3).; No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3 (WNT3) and analogues was observed.
• Sequence Length: 355

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Wingless Type MMTV Integration Site Family, Member 3 (WNT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Wingless Type MMTV Integration Site Family, Member 3 (WNT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for WNT3 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 119578109
• NCBI GeneID: 7473
• UniProt Accession #: P56703; Q2M237; Q9H1J9
• Molecular Weight: 39,645 Da
• NCBI Official Full Name: wingless-type MMTV integration site family, member 3
• NCBI Official Synonym Full Names: wingless-type MMTV integration site family, member 3
• NCBI Official Symbol: WNT3
• NCBI Official Synonym Symbols: INT4; TETAMS
• NCBI Protein Information: proto-oncogene Wnt-3
• UniProt Protein Name: Proto-oncogene Wnt-3
• Protein Family: Proto-oncogene
• UniProt Gene Name: WNT3
• UniProt Synonym Gene Names: INT4
• UniProt Entry Name: WNT3_HUMAN

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98% amino acid identity to mouse Wnt3 protein, and 84% to human WNT3A protein, another WNT gene product. The mouse studies show the requirement of Wnt3 in primary axis formation in the mouse. Studies of the gene expression suggest that this gene may play a key role in some cases of human breast, rectal, lung, and gastric cancer through activation of the WNT-beta-catenin-TCF signaling pathway. This gene is clustered with WNT15, another family member, in the chromosome 17q21 region. [provided by RefSeq, Jul 2008]

Uniprot Description

WNT3: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Defects in WNT3 are the cause of autosomal recessive tetra-amelia (ARTTRA). Tetra-amelia is a rare human genetic disorder characterized by complete absence of all four limbs and other anomalies such as craniofacial, nervous system, pulmonary, skeletal and urogenital defects. Belongs to the Wnt family.

Protein type: Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; proteinaceous extracellular matrix; endoplasmic reticulum lumen; Golgi lumen; extracellular region; plasma membrane

Molecular Function: protein domain specific binding; protein binding; frizzled binding; receptor agonist activity

Biological Process: neuron differentiation; embryonic forelimb morphogenesis; limb bud formation; axon guidance; cell fate commitment; mesoderm formation; gamete generation; cell morphogenesis; dorsal/ventral axis specification; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; negative regulation of axon extension involved in axon guidance; embryonic hindlimb morphogenesis; Spemann organizer formation at the anterior end of the primitive streak; anterior/posterior axis specification

Disease: Tetraamelia, Autosomal Recessive