Typical Testing Data/Standard Curve (for reference only)
Rat Vitamin D3 receptor (VDR) ELISA Kit | VDR elisa kit
Rat Vitamin D3 receptor (VDR) ELISA Kit
Reactivity
Rat
Synonyms
Vitamin D3 receptor (VDR); Rat Vitamin D3 receptor (VDR) ELISA Kit; VDR elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Competitive (Quantitative)
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VDR elisa kit
Intended Uses: This VDR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat VDR. This ELISA kit for research use only!
Principle of the Assay: VDR ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-VDR antibody and an VDR-HRP conjugate. The assay sample and buffer are incubated together with VDR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VDR concentration since VDR from samples and VDR-HRP conjugate compete for the anti-VDR antibody binding site. Since the number of sites is limited, as more sites are occupied by VDR from the sample, fewer sites are left to bind VDR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VDR concentration in each sample is interpolated from this standard curve.
Principle of the Assay: VDR ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-VDR antibody and an VDR-HRP conjugate. The assay sample and buffer are incubated together with VDR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the VDR concentration since VDR from samples and VDR-HRP conjugate compete for the anti-VDR antibody binding site. Since the number of sites is limited, as more sites are occupied by VDR from the sample, fewer sites are left to bind VDR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The VDR concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
48,318 Da
NCBI Official Full Name
vitamin D3 receptor
NCBI Official Synonym Full Names
vitamin D (1,25- dihydroxyvitamin D3) receptor<
NCBI Official Symbol
VDR
NCBI Protein Information
vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; nuclear receptor subfamily 1 group I member 1
UniProt Protein Name
Vitamin D3 receptor
Protein Family
UniProt Gene Name
VDR
UniProt Synonym Gene Names
NR1I1; VDR
UniProt Entry Name
VDR_BOVIN
Uniprot Description
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Recruited to promoters via its interaction with BAZ1B/WSTF which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Research Articles on VDR
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Product Notes
The Rat VDR vdr (Catalog #AAA7241577) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7241577 ELISA Kit recognizes Rat VDR. It is sometimes possible for the material contained within the vial of "Vitamin D3 receptor (VDR), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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