Goat TREM2 Polyclonal Antibody | anti-TREM2 antibody

Goat anti-TREM2 Antibody

Cat. Num. AAA420713

• Gene Names: TREM2; TREM-2; Trem2a; Trem2b; Trem2c
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: TREM2; Polyclonal Antibody; Goat anti-TREM2 Antibody; triggering receptor expressed on myeloid cells 2; TREM-2; Trem2a; Trem2b; Trem2c; OTTHUMP00000039771; triggering receptor expressed on myeloid cells 2a; TREM2 antibody; triggering receptor expressed on myeloid cells 2 antibody; TREM-2 antibody; Trem2a antibody; Trem2b antibody; Trem2c antibody; OTTHUMP00000039771 antibody; triggering receptor expressed on myeloid cells 2a antibody; anti-TREM2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: HGQKPGTHPPSELD
• Sequence Length: 230

• Applicable Applications for anti-TREM2 antibody: Peptide ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 32000.; Immunohistochemistry: In paraffin embedded Human Spleen shows staining of macrophages. Recommended concentration, 2-3ug/ml.; Western Blot: Preliminary experiments gave bands at approx 80kDa and 30kDa in Human Brain (Cerebellum) lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 25.4kDa according to NP_061838.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Immunogen: Peptide with sequence C-HGQKPGTHPPSELD, from the C Terminus of the protein sequence according to NP_061838.1.
• Epitope: C Terminus
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((2.5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 9507203
• NCBI GeneID: 54209
• NCBI Accession #: NP_061838.1
• NCBI GenBank Nucleotide #: NM_018965.3
• Molecular Weight: 24,669 Da
• NCBI Official Full Name: triggering receptor expressed on myeloid cells 2 isoform 1
• NCBI Official Synonym Full Names: triggering receptor expressed on myeloid cells 2
• NCBI Official Symbol: TREM2
• NCBI Official Synonym Symbols: TREM-2; Trem2a; Trem2b; Trem2c
• NCBI Protein Information: triggering receptor expressed on myeloid cells 2
• UniProt Protein Name: Triggering receptor expressed on myeloid cells 2
• Protein Family: Triggering receptor expressed on myeloid cells
• UniProt Gene Name: TREM2
• UniProt Synonym Gene Names: TREM-2
• UniProt Entry Name: TREM2_HUMAN

NCBI Description

This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Nov 2012]

Uniprot Description

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.1

Cellular Component: extracellular region; integral to membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: lipopolysaccharide binding; peptidoglycan binding; receptor activity

Biological Process: detection of lipopolysaccharide; detection of peptidoglycan; humoral immune response; innate immune response; phagocytosis, engulfment; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of calcium-mediated signaling; positive regulation of peptidyl-tyrosine phosphorylation; regulation of immune response

Disease: Polycystic Lipomembranous Osteodysplasia With Sclerosing Leukoencephalopathy