Typical Testing Data/Standard Curve (for reference only)
Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit | VIP elisa kit
Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit
Gene Names
Vip; vip/phi27
Reactivity
Rat
Synonyms
Vasoactive Intestinal Peptide (VIP); Rat Vasoactive Intestinal Peptide (VIP) ELISA Kit; PHM27; Intestinal peptide PHV-42; Peptide histidine valine 42; Peptide histidine methioninamide 27; VIP elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of VIP. No significant cross-reactivity or interference between VIP and analogues was observed.
Sequence Length
170
Samples
Serum, plasma and other biological fluids.
Assay Type
Competitive inhibition
Detection Range
7.812-500pg/mL
Sensitivity
2.64pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level VIP were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level VIP were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for VIP elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of VIP in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VIP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VIP and unlabeled VIP (Standards or samples) with the pre-coated antibody specific to VIP. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VIP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VIP in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to VIP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled VIP and unlabeled VIP (Standards or samples) with the pre-coated antibody specific to VIP. After incubation the unbound conjugate is washed off. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of VIP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of VIP in the sample.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,079 Da
NCBI Official Full Name
VIP peptides preproprotein
NCBI Official Synonym Full Names
vasoactive intestinal peptide
NCBI Official Symbol
Vip
NCBI Official Synonym Symbols
vip/phi27
NCBI Protein Information
VIP peptides
UniProt Protein Name
VIP peptides
Protein Family
UniProt Gene Name
Vip
UniProt Synonym Gene Names
VIP
NCBI Description
peptide that causes vasodilation [RGD, Feb 2006]
Uniprot Description
VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder.PHM-27 is a potent agonist of the calcitonin receptor CALCR, with similar efficacy as calcitonin (). PHI also causes vasodilation.
Research Articles on VIP
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Product Notes
The Rat VIP vip (Catalog #AAA452263) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA452263 ELISA Kit recognizes Rat VIP. It is sometimes possible for the material contained within the vial of "Vasoactive Intestinal Peptide (VIP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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