Rat Vascular Endothelial cell Growth Factor A, VEGF-A ELISA Kit | VEGF-A elisa kit

Rat Vascular Endothelial cell Growth Factor A, VEGF-A ELISA Kit

Cat. Num. AAA165440

• Gene Names: VEGFA; VEGF
• Reactivity: Rat
• Synonyms: Vascular Endothelial cell Growth Factor A; VEGF-A; Rat Vascular Endothelial cell Growth Factor A; VEGF-A ELISA Kit; VEGF-A elisa kit

• Reactivity: Rat
• Sequence Length: 146

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 5ng/L - 600ng/L
• Sensitivity: 2.11ng/L
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for VEGF-A elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Vascular Endothelial Cell Growth Factor A (also known as VEGF-A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat VEGF-A antibody. VEGF-A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat VEGF-A Antibody is added and binds to VEGF-A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated VEGF-A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat VEGF-A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 68341933
• NCBI GeneID: 443103
• NCBI Accession #: NP_001020281.1
• NCBI GenBank Nucleotide #: NM_001025110.1
• UniProt Accession #: P50412
• Molecular Weight: 17,247 Da
• NCBI Official Full Name: vascular endothelial growth factor A
• NCBI Official Symbol: VEGFA
• NCBI Official Synonym Symbols: VEGF
• NCBI Protein Information: vascular endothelial growth factor A
• UniProt Protein Name: Vascular endothelial growth factor A
• UniProt Gene Name: VEGFA
• UniProt Synonym Gene Names: VEGF; VEGF-A; VPF

Uniprot Description

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin (). Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development ().