Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor ligand superfamily member 15 (TNFSF15) Standard Curve.)
Rat Tumor Necrosis Factor Ligand Superfamily Member 15 (TNFSF15) ELISA Kit | TNFSF15 elisa kit
Rat Tumor Necrosis Factor Ligand Superfamily Member 15 (TNFSF15) ELISA Kit
Gene Names
Tnfsf15; Tnlg1b
Reactivity
Rat
Synonyms
Tumor Necrosis Factor Ligand Superfamily Member 15 (TNFSF15); Rat Tumor Necrosis Factor Ligand Superfamily Member 15 (TNFSF15) ELISA Kit; TNFSF15; MGC129934; MGC129935; TL1; TL1A; VEGI; VEGI192A; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor-192A; TNFSF15 elisa kit
Reactivity
Rat
Specificity
Rat Tumor necrosis factor ligand superfamily member 15 (TNFSF15) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFSF15. No significant cross-reactivity or interference between Rat TNFSF15 and analogues was observed.
Sequence Length
252
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor ligand superfamily member 15 (TNFSF15) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Tumor necrosis factor ligand superfamily member 15 (TNFSF15) Standard Curve.)
Related Product Information for TNFSF15 elisa kit
Description: This Rat Tumor necrosis factor ligand superfamily member 15 (TNFSF15) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF15 in samples. An antibody specific for TNFSF15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFSF15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF15 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF15 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: VEGI is an anti-angiogenic protein. It belongs to tumor necrosis factor (ligand) superfamily, where it is member 15. The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.
Background: VEGI is an anti-angiogenic protein. It belongs to tumor necrosis factor (ligand) superfamily, where it is member 15. The protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 15
NCBI Official Synonym Full Names
TNF superfamily member 15
NCBI Official Symbol
Tnfsf15
NCBI Official Synonym Symbols
Tnlg1b
NCBI Protein Information
tumor necrosis factor ligand superfamily member 15
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 15
Protein Family
UniProt Gene Name
Tnfsf15
UniProt Synonym Gene Names
Tl1; Vegi
UniProt Entry Name
TNF15_RAT
NCBI Description
ligand for DR3 and TR6/DcR3; activates T cells leading to survival and secretion of proinflammatory cytokines; promotes acute graft-versus-host reactions [RGD, Feb 2006]
Uniprot Description
Function: Receptor for TNFRSF25 and TNFRSF6B. Mediates activation of NF-kappa-B. Inhibits vascular endothelial growth and angiogenesis (in vitro). Promotes activation of caspases and apoptosis. Promotes splenocyte alloactivation
Research Articles on TNFSF15
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Product Notes
The Rat TNFSF15 tnfsf15 (Catalog #AAA9711669) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711669 ELISA Kit recognizes Rat TNFSF15. It is sometimes possible for the material contained within the vial of "Tumor Necrosis Factor Ligand Superfamily Member 15 (TNFSF15), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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