Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane 4 L6 family member 4 (TM4SF4) Standard Curve.)
Rat Transmembrane 4 L6 Family Member 4 (TM4SF4) ELISA Kit | TM4SF4 elisa kit
Rat Transmembrane 4 L6 Family Member 4 (TM4SF4) ELISA Kit
Gene Names
Tm4sf4; Lrtm4
Reactivity
Rat
Synonyms
Transmembrane 4 L6 Family Member 4 (TM4SF4); Rat Transmembrane 4 L6 Family Member 4 (TM4SF4) ELISA Kit; TM4SF4; FLJ31015; ILTMP; il-TMP; intestinal and liver (il) tetraspan membrane protein; transmembrane 4 superfamily member 4; TM4SF4 elisa kit
Reactivity
Rat
Specificity
Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TM4SF4. No significant cross-reactivity or interference between Rat TM4SF4 and analogues was observed.
Sequence Length
202
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane 4 L6 family member 4 (TM4SF4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Transmembrane 4 L6 family member 4 (TM4SF4) Standard Curve.)
Related Product Information for TM4SF4 elisa kit
Description: This Rat Transmembrane 4 L6 family member 4 (TM4SF4) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF4 in samples. An antibody specific for TM4SF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TM4SF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: TM4SF4 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that can regulate cell proliferation. The use of alternate polyadenylation sites has been found for this gene. Sequence analysis predicted that the 202-amino acid tetraspan membrane protein, which is 50% identical to TM4SF1, contains 4 hydrophobic transmembrane domains and a hydrophilic region between domains 3 and 4 that contains 2 potential N-linked glycosylation sites.
Background: TM4SF4 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that can regulate cell proliferation. The use of alternate polyadenylation sites has been found for this gene. Sequence analysis predicted that the 202-amino acid tetraspan membrane protein, which is 50% identical to TM4SF1, contains 4 hydrophobic transmembrane domains and a hydrophilic region between domains 3 and 4 that contains 2 potential N-linked glycosylation sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transmembrane 4 L6 family member 4
NCBI Official Synonym Full Names
transmembrane 4 L six family member 4
NCBI Official Symbol
Tm4sf4
NCBI Official Synonym Symbols
Lrtm4
NCBI Protein Information
transmembrane 4 L6 family member 4
UniProt Protein Name
Transmembrane 4 L6 family member 4
Protein Family
UniProt Gene Name
Tm4sf4
UniProt Synonym Gene Names
Lrtm4
UniProt Entry Name
T4S4_RAT
NCBI Description
member of tetraspanin superfamily; may mediate cell proliferation and liver regeneration [RGD, Feb 2006]
Research Articles on TM4SF4
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Product Notes
The Rat TM4SF4 tm4sf4 (Catalog #AAA9711713) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711713 ELISA Kit recognizes Rat TM4SF4. It is sometimes possible for the material contained within the vial of "Transmembrane 4 L6 Family Member 4 (TM4SF4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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