Rat Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit | TAN1 elisa kit

Rat Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit

Cat. Num. AAA2098095

• Gene Names: NOTCH1; hN1; AOS5; TAN1; AOVD1
• Reactivity: Rat
• Synonyms: Translocation Associated Notch Homolog 1 (TAN1); Rat Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit; NOTCH1; hN1; NEXT; NICD; Translocation-associated notch protein TAN-1; TAN1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Translocation Associated Notch Homolog 1 (TAN1).; No significant cross-reactivity or interference between Translocation Associated Notch Homolog 1 (TAN1) and analogues was observed.
• Sequence Length: 2444

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.066ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocation Associated Notch Homolog 1 (TAN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocation Associated Notch Homolog 1 (TAN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocation Associated Notch Homolog 1 (TAN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocation Associated Notch Homolog 1 (TAN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocation Associated Notch Homolog 1 (TAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocation Associated Notch Homolog 1 (TAN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TAN1 elisa kit

Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 338675
• NCBI GeneID: 4851
• Molecular Weight: 272,505 Da
• NCBI Official Full Name: TAN1, partial
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: NOTCH1
• NCBI Official Synonym Symbols: hN1; AOS5; TAN1; AOVD1
• NCBI Protein Information: neurogenic locus notch homolog protein 1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: tRNA acetyltransferase
• UniProt Gene Name: NOTCH1
• UniProt Synonym Gene Names: TAN1; Notch 1; hN1; NEXT; NICD

NCBI Description

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Uniprot Description

Notch 1: Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds. Interacts with DNER, DTX1, DTX2 and RBPJ/RBPSUH. Also interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH1. The activated membrane-bound form interacts with AAK1 which promotes NOTCH1 stabilization. Forms a trimeric complex with FBXW7 and SGK1. Interacts with HIF1AN. HIF1AN negatively regulates the function of notch intracellular domain (NICD), accelerating myogenic differentiation. In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues. Belongs to the NOTCH family.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Oncoprotein; Receptor, misc.; Transcription factor

Chromosomal Location of Human Ortholog: 9q34.3

Cellular Component: acrosomal vesicle; adherens junction; apical plasma membrane; cell surface; cytosol; endoplasmic reticulum membrane; extracellular region; Golgi membrane; integral component of membrane; nucleoplasm; nucleus; plasma membrane; receptor complex

Molecular Function: calcium ion binding; chromatin DNA binding; DNA binding transcription factor activity; enzyme binding; enzyme inhibitor activity; Notch binding; protein binding; protein heterodimerization activity; sequence-specific DNA binding; transmembrane receptor activity

Biological Process: activation of Notch receptor target transcription factor; aortic valve morphogenesis; apoptotic process involved in embryonic digit morphogenesis; astrocyte differentiation; atrioventricular node development; atrioventricular valve morphogenesis; auditory receptor cell fate commitment; axonogenesis; branching morphogenesis of a tube; cardiac atrium morphogenesis; cardiac chamber formation; cardiac left ventricle morphogenesis; cardiac muscle cell proliferation; cardiac muscle morphogensis; cardiac right atrium morphogenesis; cardiac right ventricle formation; cardiac ventricle morphogenesis; cell cycle arrest; cell differentiation in spinal cord; cell fate specification; cell migration involved in endocardial cushion formation; cellular response to follicle-stimulating hormone stimulus; collecting duct development; compartment pattern specification; coronary vein morphogenesis; determination of left/right symmetry; distal tubule development; embryonic hindlimb morphogenesis; endocardial cushion morphogenesis; endocardium development; endocardium morphogenesis; endoderm development; epithelial to mesenchymal transition; epithelial to mesenchymal transition involved in endocardial cushion formation; forebrain development; foregut morphogenesis; glomerular mesangial cell development; growth involved in heart morphogenesis; hair follicle morphogenesis; heart development; heart looping; humoral immune response; immune response; in utero embryonic development; inflammatory response to antigenic stimulus; interleukin-4 secretion; keratinocyte differentiation; left/right axis specification; liver development; lumen formation; lung development; mesenchymal cell development; mitral valve formation; negative regulation of anoikis; negative regulation of auditory receptor cell differentiation; negative regulation of BMP signaling pathway; negative regulation of calcium ion-dependent exocytosis; negative regulation of canonical Wnt signaling pathway; negative regulation of catalytic activity; negative regulation of cell migration involved in sprouting angiogenesis; negative regulation of cell proliferation; negative regulation of cell-substrate adhesion; negative regulation of endothelial cell chemotaxis; negative regulation of gene expression; negative regulation of growth rate; negative regulation of myoblast differentiation; negative regulation of myotube differentiation; negative regulation of neurogenesis; negative regulation of oligodendrocyte differentiation; negative regulation of ossification; negative regulation of osteoblast differentiation; negative regulation of photoreceptor cell differentiation; negative regulation of pro-B cell differentiation; negative regulation of stem cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube development; Notch signaling pathway; Notch signaling pathway involved in regulation of secondary heart field cardioblast proliferation; oligodendrocyte differentiation; organ regeneration; pericardium morphogenesis; positive regulation of aorta morphogenesis; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of BMP signaling pathway; positive regulation of cardiac muscle cell proliferation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of endothelial cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of epithelial to mesenchymal transition; positive regulation of ERK1 and ERK2 cascade; positive regulation of JAK-STAT cascade; positive regulation of keratinocyte differentiation; positive regulation of neuroblast proliferation; positive regulation of Notch signaling pathway; positive regulation of Ras protein signal transduction; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of viral genome replication; pulmonary valve morphogenesis; regulation of extracellular matrix assembly; regulation of somitogenesis; regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation; regulation of transcription, DNA-templated; response to corticosteroid stimulus; response to lipopolysaccharide; response to muramyl dipeptide; somatic stem cell division; spermatogenesis; sprouting angiogenesis; tissue regeneration; transcription initiation from RNA polymerase II promoter; ventricular trabecula myocardium morphogenesis

Disease: Adams-oliver Syndrome 5; Aortic Valve Disease 1