Human Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit | TAN1 elisa kit

Human Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit

Cat. Num. AAA2706227

• Gene Names: NOTCH1; hN1; AOS5; TAN1; AOVD1
• Reactivity: Human
• Synonyms: Translocation Associated Notch Homolog 1 (TAN1); Human Translocation Associated Notch Homolog 1 (TAN1) ELISA Kit; NOTCH1; hN1; NEXT; NICD; Translocation-associated notch protein TAN-1; TAN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Translocation Associated Notch Homolog 1 (TAN1).; No significant cross-reactivity or interference between Translocation Associated Notch Homolog 1 (TAN1) and analogues was observed.
• Sequence Length: 2,555

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.062ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Translocation Associated Notch Homolog 1 (TAN1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Translocation Associated Notch Homolog 1 (TAN1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TAN1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Translocation Associated Notch Homolog 1 (TAN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Translocation Associated Notch Homolog 1 (TAN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Translocation Associated Notch Homolog 1 (TAN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Translocation Associated Notch Homolog 1 (TAN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148833508
• NCBI GeneID: 4851
• NCBI Accession #: NP_060087.3
• NCBI GenBank Nucleotide #: NM_017617.4
• UniProt Accession #: P46531; Q59ED8; Q5SXM3
• Molecular Weight: 272,505 Da
• NCBI Official Full Name: neurogenic locus notch homolog protein 1 preproprotein
• NCBI Official Synonym Full Names: notch 1
• NCBI Official Symbol: NOTCH1
• NCBI Official Synonym Symbols: hN1; AOS5; TAN1; AOVD1
• NCBI Protein Information: neurogenic locus notch homolog protein 1
• UniProt Protein Name: Neurogenic locus notch homolog protein 1
• Protein Family: tRNA acetyltransferase
• UniProt Gene Name: NOTCH1
• UniProt Synonym Gene Names: TAN1; Notch 1; hN1; NEXT; NICD

NCBI Description

This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma. [provided by RefSeq, Jan 2016]

Uniprot Description

Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. Involved in angiogenesis; negatively regulates endothelial cell proliferation and migration and angiogenic sprouting. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. Important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, functions as a receptor for neuronal DNER and is involved in the differentiation of Bergmann glia. Represses neuronal and myogenic differentiation. May play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation. May be involved in mesoderm development, somite formation and neurogenesis. May enhance HIF1A function by sequestering HIF1AN away from HIF1A. Required for the THBS4 function in regulating protective astrogenesis from the subventricular zone (SVZ) niche after injury. Involved in determination of left/right symmetry by modulating the balance between motile and immotile (sensory) cilia at the left-right organiser (LRO).