Typical Testing Data/Standard Curve
Rat Transcription factor HES-1 (HES1) ELISA Kit | HES1 elisa kit
Rat Transcription factor HES-1 (HES1) ELISA Kit
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve
Principle of the Assay||HES1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-HES1 antibody and an HES1 -HRP conjugate. The assay sample and buffer are incubated together with HES1 -HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HES1 concentration since HES1 from samples and HES1 -HRP conjugate compete for the anti-HES1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HES1 from the sample, fewer sites are left to bind HES1 -HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HES1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. [provided by RefSeq, Jul 2008]
Uniprot Description
HES1: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity. May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.
Protein type: Transcription factor; Cell development/differentiation; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 3q28-q29
Cellular Component: nucleoplasm; cytoplasm; nucleus
Molecular Function: protein binding; protein homodimerization activity; DNA binding; sequence-specific DNA binding; histone deacetylase binding; chaperone binding; transcription factor activity; transcription factor binding
Biological Process: transcription from RNA polymerase II promoter; radial glial cell differentiation in the forebrain; midbrain-hindbrain boundary morphogenesis; hindbrain morphogenesis; somatic stem cell maintenance; regulation of fat cell differentiation; regulation of timing of neuron differentiation; cell maturation; positive regulation of JAK-STAT cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; positive regulation of cell proliferation; midbrain development; adenohypophysis development; protein complex assembly; positive regulation of T cell proliferation; trochlear nerve development; oculomotor nerve development; cell adhesion; positive regulation of mitotic cell cycle, embryonic; positive regulation of Notch signaling pathway; positive regulation of BMP signaling pathway; positive regulation of DNA binding; positive regulation of astrocyte differentiation; STAT protein nuclear translocation; nervous system development; cell migration; smoothened signaling pathway; Notch signaling pathway; thymus development; auditory receptor cell fate determination; liver development; endocrine pancreas development; negative regulation of oligodendrocyte differentiation; telencephalon development; lateral inhibition; negative regulation of auditory receptor cell differentiation; neuron morphogenesis during differentiation; artery morphogenesis; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; lung development
Research Articles on HES1
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Product Notes
The Rat HES1 hes1 (Catalog #AAA7229266) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7229266 ELISA Kit recognizes Rat HES1. It is sometimes possible for the material contained within the vial of "Transcription factor HES-1 (HES1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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