Standard Curve (Sample)
Rat Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1) ELISA Kit | ALDH3A1 elisa kit
Rat Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1) ELISA Kit
Gene Names
ALDH3A1; ALDH3; ALDHIII
Reactivity
Rat
Synonyms
Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1); N/A; Rat Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1) ELISA Kit; ALDH3A1 elisa kit
Reactivity
Rat
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Standard Curve (Sample)
Standard Curve (Sample)
Related Product Information for ALDH3A1 elisa kit
Intended Uses: This ALDH3A1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ALDH3A1 This ELISA kit for research use only!
Principle of the Assay: ALDH3A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALDH3A1 antibody and an ALDH3A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH3A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH3A1 concentration since ALDH3A1 from samples and ALDH3A1-HRP conjugate compete for the anti-ALDH3A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH3A1 from the sample, fewer sites are left to bind ALDH3A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH3A1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: ALDH3A1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ALDH3A1 antibody and an ALDH3A1-HRP conjugate. The assay sample and buffer are incubated together with ALDH3A1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ALDH3A1 concentration since ALDH3A1 from samples and ALDH3A1-HRP conjugate compete for the anti-ALDH3A1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ALDH3A1 from the sample, fewer sites are left to bind ALDH3A1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ALDH3A1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,395 Da
NCBI Official Full Name
aldehyde dehydrogenase, dimeric NADP-preferring
NCBI Official Synonym Full Names
aldehyde dehydrogenase 3 family, member A1
NCBI Official Symbol
ALDH3A1
NCBI Official Synonym Symbols
ALDH3; ALDHIII
NCBI Protein Information
aldehyde dehydrogenase, dimeric NADP-preferring; stomach aldehyde dehydrogenase; aldehyde dehydrogenase type III; aldehyde dehydrogenase isozyme 3
UniProt Protein Name
Aldehyde dehydrogenase, dimeric NADP-preferring
UniProt Gene Name
ALDH3A1
UniProt Synonym Gene Names
ALDH3
UniProt Entry Name
AL3A1_HUMAN
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Product Notes
The Rat ALDH3A1 aldh3a1 (Catalog #AAA244593) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA244593 ELISA Kit recognizes Rat ALDH3A1. It is sometimes possible for the material contained within the vial of "Aldehyde dehydrogenase, dimeric NADP-preferring (ALDH3A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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