RPRD1B recombinant protein

RPRD1B Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121614

• Purity: >85% as determined by SDS-PAGE
• Synonyms: RPRD1B; RPRD1B Protein; Human; Recombinant (His Tag); Human RPRD1B Protein (His Tag); C20orf77 Protein; CREPT Protein; dJ1057B20.2 Protein; NET60 Protein; regulation of nuclear pre-mRNA domain containing 1B; RPRD1B recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asp326

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human RPRD1B (AAH33629.1)(Met1-Asp326) was expressed with an N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for RPRD1B recombinant protein

Background: RPRD1B, together with RPRD1A, can accompany RNAP II from promoter regions to 3'-untranslated regions during transcription in vivo, predominantly interact with phosphorylated RNAP II, and can reduce CTD S5- and S7-phosphorylated RNAP II at target gene promoters. RNA polymerase II C-terminal domain (CTD) phosphorylation is important for various transcription-related processes. RPRD1B is a transcriptional regulator that enhances expression of CCND1. It also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A.

References

Ni Z. et al., 2011, Transcription. 2 (5): 237-42. Kristensen AR. et al., 2012, Nat Methods. 9 (9): 907-9. Woods NT. et al., 2012, Sci Signal.5 (242): rs6.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human RPRD1B comprises 346 amino acids and has a predicted molecular mass of 39.3kDa. The apparent molecular mass of the protein is approximately 39kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein Family: Regulation of nuclear pre-mRNA domain-containing protein