Typical Testing Data/Standard Curve (for reference only)
Rat TGF-beta3 ELISA Kit | TGF-beta3 elisa kit
Rat TGF-beta3 (Transforming Growth Factor Beta 3) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TGF-beta3. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Rat TGF-beta3 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TGF-beta3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Rat TGF-beta3. You can calculate the concentration of Rat TGF-beta3 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the TGF-beta family of proteins. The encoded protein is secreted and is involved in embryogenesis and cell differentiation. Defects in this gene are a cause of familial arrhythmogenic right ventricular dysplasia 1. [provided by RefSeq, Mar 2009]
Uniprot Description
TGFB3: Involved in embryogenesis and cell differentiation. Homodimer; disulfide-linked. Interacts with ASPN. Belongs to the TGF-beta family.
Protein type: Secreted, signal peptide; Secreted; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Cell development/differentiation
Chromosomal Location of Human Ortholog: 14q24
Cellular Component: extracellular matrix; extracellular space; cell surface; cell soma; T-tubule; extracellular region; plasma membrane; nucleus
Molecular Function: identical protein binding; protein binding; growth factor activity; protein heterodimerization activity; transforming growth factor beta binding; punt binding; cytokine activity
Biological Process: extracellular matrix organization and biogenesis; positive regulation of apoptosis; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of collagen biosynthetic process; female pregnancy; palate development; SMAD protein nuclear translocation; regulation of apoptosis; odontogenesis; negative regulation of cell proliferation; platelet degranulation; mammary gland development; transforming growth factor beta receptor signaling pathway; salivary gland morphogenesis; embryonic neurocranium morphogenesis; negative regulation of neuron apoptosis; cell growth; inner ear development; aging; positive regulation of filopodium formation; uterine wall breakdown; platelet activation; intercellular junction assembly and maintenance; in utero embryonic development; positive regulation of bone mineralization; regulation of cell proliferation; gut development; positive regulation of protein secretion; response to estrogen stimulus; negative regulation of DNA replication; positive regulation of cell division; regulation of MAPKKK cascade; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; response to progesterone stimulus; negative regulation of transforming growth factor beta receptor signaling pathway; alveolus development; positive regulation of DNA replication
Disease: Arrhythmogenic Right Ventricular Dysplasia, Familial, 1; Rienhoff Syndrome
Research Articles on TGF-beta3
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Product Notes
The Rat TGF-beta3 tgfb3 (Catalog #AAA2515124) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515124 ELISA Kit recognizes Rat TGF-beta3. It is sometimes possible for the material contained within the vial of "TGF-beta3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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