Rat Tau Protein (MAPT) ELISA Kit | MAPT elisa kit

Rat Tau Protein (MAPT) High Sensitive ELISA Kit

Cat. Num. AAA2708087

• Gene Names: Mapt; Tau; pTau; Mtapt; RNPTAU
• Reactivity: Rat
• Applications: ELISA
• Synonyms: Tau Protein (MAPT); Rat Tau Protein (MAPT) High Sensitive ELISA Kit; Tau Protein; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Microtubule Associated Protein Tau; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; MAPT elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Tau Protein (MAPT).; No significant cross-reactivity or interference between High Sensitive Tau Protein (MAPT) and analogues was observed.
• Sequence Length: 752

• Applicable Applications for MAPT elisa kit: ELISA (EIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 7.8-500pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.9pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Tau Protein (MAPT) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Tau Protein (MAPT) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Tau Protein (MAPT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Tau Protein (MAPT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Tau Protein (MAPT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Tau Protein (MAPT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 13432197
• NCBI GeneID: 29477
• UniProt Accession #: P19332
• NCBI Official Full Name: Microtubule-associated protein tau
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; pTau; Mtapt; RNPTAU
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau
• UniProt Entry Name: TAU_RAT

NCBI Description

a microtubule-associated protein; expression is found specifically in neurons [RGD, Feb 2006]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: microtubule; neuron projection; cell; postsynaptic density; cytosol; microtubule cytoskeleton; growth cone; axon; tubulin complex; cytoplasm; plasma membrane; axoneme; intracellular; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; protein phosphatase 2A binding; microtubule binding; protein complex binding; apolipoprotein binding; protein kinase binding; SH3 domain binding

Biological Process: axon extension; apoptosis; positive regulation of microtubule polymerization; positive regulation of axon extension; axon cargo transport; neuron migration; female pregnancy; microtubule cytoskeleton organization and biogenesis; adult walking behavior; microtubule-based process; mitochondrion transport along microtubule; response to organic substance; neuron differentiation; axonogenesis; negative regulation of intracellular transport; regulation of autophagy; brain development; induction of apoptosis by oxidative stress; response to nutrient