Rat Synapsin II (SYN2) ELISA Kit | SYN2 elisa kit

Rat Synapsin II (SYN2) ELISA Kit

Cat. Num. AAA2098027

• Gene Names: SYN2; SYNII
• Reactivity: Rat
• Synonyms: Synapsin II (SYN2); Rat Synapsin II (SYN2) ELISA Kit; SYNII; SYNIIa; SYNIIb; SYN2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Synapsin II (SYN2).; No significant cross-reactivity or interference between Synapsin II (SYN2) and analogues was observed.
• Sequence Length: 582

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 27pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synapsin II (SYN2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synapsin II (SYN2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SYN2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synapsin II (SYN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synapsin II (SYN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synapsin II (SYN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synapsin II (SYN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SYN2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 19924103
• NCBI GeneID: 6854
• NCBI Accession #: NP_598328.1
• NCBI GenBank Nucleotide #: NM_133625.4
• UniProt Accession #: Q92777; A8MY98
• Molecular Weight: 52,162 Da
• NCBI Official Full Name: synapsin-2 isoform IIa
• NCBI Official Synonym Full Names: synapsin II
• NCBI Official Symbol: SYN2
• NCBI Official Synonym Symbols: SYNII
• NCBI Protein Information: synapsin-2
• UniProt Protein Name: Synapsin-2
• Protein Family: Synapsin
• UniProt Gene Name: SYN2

NCBI Description

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family encodes a neuron-specific phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal. Polymorphisms in this gene are associated with abnormal presynaptic function and related neuronal disorders, including autism, epilepsy, bipolar disorder and schizophrenia. Alternative splicing of this gene results in multiple transcript variants. The tissue inhibitor of metalloproteinase 4 gene is located within an intron of this gene and is transcribed in the opposite direction. [provided by RefSeq, Feb 2014]

Uniprot Description

SYN2: neuronal phosphoprotein that selectively binds to small synaptic vesicles in the presynaptic nerve terminal and to the cytoskeleton. May function in the regulation of neurotransmitter release and synaptogenesis. Mutations in this gene may be associated with abnormal presynaptic function and schizophrenia. Alternative splicing results in two isoforms.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 3p25.2

Cellular Component: cell junction; synapse; synaptic vesicle membrane

Molecular Function: ATP binding; catalytic activity

Biological Process: neurotransmitter secretion; synaptic transmission

Disease: Schizophrenia