Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit | SREBF1 elisa kit

Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit

Cat. Num. AAA457193

• Gene Names: Srebf1; ADD1; ADD-1; Srebp1; SREBP-1; SREBP-1c
• Reactivity: Rat
• Synonyms: Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1); Rat Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1) ELISA Kit; SREBP1; Sterol Regulatory Element-Binding Protein 1; Adipocyte determination- and differentiation-dependent factor 1; ADD1; SREBF1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of SPB. No significant cross-reactivity or interference between SPB and analogues was observed.
• Sequence Length: 1134

• Samples: Mouse Serum, Plasma, Tissue Homogenates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: 0.33ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SPB were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SPB were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SREBF1 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of SPB in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SPB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SPB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SPB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 453040303
• NCBI GeneID: 78968
• NCBI Accession #: NP_001263636.1
• NCBI GenBank Nucleotide #: NM_001276707.1
• UniProt Accession #: P56720; Q99PI6; Q99PI7
• Molecular Weight: 118,027 Da
• NCBI Official Full Name: sterol regulatory element-binding protein 1 isoform 1
• NCBI Official Synonym Full Names: sterol regulatory element binding transcription factor 1
• NCBI Official Symbol: Srebf1
• NCBI Official Synonym Symbols: ADD1; ADD-1; Srebp1; SREBP-1; SREBP-1c
• NCBI Protein Information: sterol regulatory element-binding protein 1
• UniProt Protein Name: Sterol regulatory element-binding protein 1
• UniProt Gene Name: Srebf1
• UniProt Synonym Gene Names: Srebp1; SREBP-1; ADD1

NCBI Description

transcription factor; binds to the sterol regulatory element 1; regulates the transcription of genes important for sterol biosynthesis [RGD, Feb 2006]

Uniprot Description

Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') (PubMed:7739539). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').