Typical Testing Data/Standard Curve (for reference only)
Rat Smoothened (SMO) ELISA Kit | SMO elisa kit
Rat Smoothened (SMO) ELISA Kit
Gene Names
SMO; Gx; CRJS; SMOH; FZD11
Reactivity
Rat
Synonyms
Smoothened (SMO); Rat Smoothened (SMO) ELISA Kit; Gx; SMOH; SMO elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Slit2. No significant cross-reactivity or interference between Slit2 and analogues was observed.
Sequence Length
787
Application Notes
Enzyme-linked immunosorbent assay for Antigen Detection.
Samples
Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
Assay Type
Quantitative Sandwich
Detection Range
0.312-20ng/mL
Sensitivity
0.122ng/mL.
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Slit2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Slit2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for SMO elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Slit2 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Slit2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Slit2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slit2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Slit2. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Slit2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Slit2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Slit2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for SMO elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
86,397 Da
NCBI Official Full Name
smoothened
NCBI Official Synonym Full Names
smoothened, frizzled class receptor
NCBI Official Symbol
SMO
NCBI Official Synonym Symbols
Gx; CRJS; SMOH; FZD11
NCBI Protein Information
smoothened homolog
UniProt Protein Name
Smoothened homolog
Protein Family
UniProt Gene Name
SMO
UniProt Synonym Gene Names
SMOH; SMO
NCBI Description
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex. [provided by RefSeq, Jul 2010]
Uniprot Description
G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia (PubMed:19592253). Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation ().
Research Articles on SMO
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Product Notes
The Rat SMO smo (Catalog #AAA2104901) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2104901 ELISA Kit recognizes Rat SMO. Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the SMO smo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smoothened (SMO), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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