Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Slit homolog 3 protein (SLIT3) Standard Curve.)
Rat Slit Homolog 3 Protein (SLIT3) ELISA Kit | SLIT3 elisa kit
Rat Slit Homolog 3 Protein (SLIT3) ELISA Kit
Gene Names
Slit3; Megf5
Reactivity
Rat
Synonyms
Slit Homolog 3 Protein (SLIT3); Rat Slit Homolog 3 Protein (SLIT3) ELISA Kit; SLIT3; FLJ10764; MEGF5; SLIL2; SLIT1; Slit-3; slit2; slit homolog 3; SLIT3 elisa kit
Reactivity
Rat
Specificity
Rat Slit homolog 3 protein (SLIT3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLIT3. No significant cross-reactivity or interference between Rat SLIT3 and analogues was observed.
Sequence Length
1523
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Slit homolog 3 protein (SLIT3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Slit homolog 3 protein (SLIT3) Standard Curve.)
Related Product Information for SLIT3 elisa kit
Description: This Rat Slit homolog 3 protein (SLIT3) ELISA Kit employs a two-site sandwich ELISA to quantitate SLIT3 in samples. An antibody specific for SLIT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLIT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLIT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLIT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
Background: The SLIT3 cDNA encodes a 1,523-amino acid polypeptide with 41.1% similarity to the Drosophila 'slit' protein. Northern blot analysis revealed that the human SLIT3 gene was expressed as a major 5.5- and a minor 9.5-kb transcript primarily in the thyroid. SLIT1 and SLIT2 mRNAs were primarily expressed in the brain and spinal cord, respectively. In situ hybridization studies indicated that the rat Slit1 mRNA was specifically expressed in the neurons of fetal and adult forebrains. These data suggested that the SLIT genes form an evolutionarily conserved group in vertebrates and invertebrates, and that the mammalian SLIT proteins may participate in the formation and maintenance of the nervous and endocrine systems by protein-protein interactions.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
slit homolog 3 protein
NCBI Official Synonym Full Names
slit guidance ligand 3
NCBI Official Symbol
Slit3
NCBI Official Synonym Symbols
Megf5
NCBI Protein Information
slit homolog 3 protein
UniProt Protein Name
Slit homolog 3 protein
Protein Family
UniProt Gene Name
Slit3
UniProt Synonym Gene Names
Megf5; Slit-3; Multiple EGF-like domains protein 5
NCBI Description
localized to mitochondria; has less conservation with Drosophila slit than family members slit1 and slit2; may play a role in neurogenesis [RGD, Feb 2006]
Uniprot Description
May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
Research Articles on SLIT3
Similar Products
Product Notes
The Rat SLIT3 slit3 (Catalog #AAA9711857) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711857 ELISA Kit recognizes Rat SLIT3. It is sometimes possible for the material contained within the vial of "Slit Homolog 3 Protein (SLIT3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
