Human SLIT3 (Slit homolog 3 protein) ELISA Kit | SLIT3 elisa kit

Human SLIT3 (Slit homolog 3 protein) ELISA Kit

Cat. Num. AAA7612259

• Gene Names: SLIT3; MEGF5; SLIL2; SLIT1; slit2; Slit-3
• Reactivity: Human
• Synonyms: SLIT3 (Slit homolog 3 protein); Human SLIT3 (Slit homolog 3 protein) ELISA Kit; Slit homolog 3 protein; Slit-3; Multiple epidermal growth factor-like domains protein 5; Multiple EGF-like domains protein 5; SLIT3; KIAA0814; MEGF5; SLIL2; UNQ691; PRO1336; SLIT3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SLIT3. No significant cross-reactivity or interference between SLIT3 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SLIT3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 11321571
• NCBI GeneID: 6586
• NCBI Accession #: NP_003053.1
• NCBI GenBank Nucleotide #: NM_003062.3
• UniProt Accession #: O75094; O95804; Q9UFH5; A6H8U9; J3KNP3
• Molecular Weight: 167,713 Da
• NCBI Official Full Name: slit homolog 3 protein isoform 2
• NCBI Official Synonym Full Names: slit homolog 3 (Drosophila)
• NCBI Official Symbol: SLIT3
• NCBI Official Synonym Symbols: MEGF5; SLIL2; SLIT1; slit2; Slit-3
• NCBI Protein Information: slit homolog 3 protein; multiple EGF-like domains protein 5; multiple epidermal growth factor-like domains protein 5
• UniProt Protein Name: Slit homolog 3 protein
• Protein Family: Slit homolog 3 protein
• UniProt Gene Name: SLIT3
• UniProt Synonym Gene Names: KIAA0814; MEGF5; SLIL2; Slit-3
• UniProt Entry Name: SLIT3_HUMAN

NCBI Description

The protein encoded by this gene is secreted, likely interacting with roundabout homolog receptors to effect cell migration. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

SLIT3: May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q35

Cellular Component: extracellular space; mitochondrion

Molecular Function: Roundabout binding; calcium ion binding

Biological Process: axon extension involved in axon guidance; chemorepulsion involved in embryonic olfactory bulb interneuron migration; axon guidance; negative regulation of cell proliferation; organ morphogenesis; response to cortisol stimulus; negative chemotaxis; negative regulation of cell growth; cellular response to hormone stimulus