Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Securin (PTTG1) Standard Curve.)
Rat Securin (PTTG1) ELISA Kit | PTTG1 elisa kit
Rat Securin (PTTG1) ELISA Kit
Gene Names
Pttg1; Pttg
Reactivity
Rat
Synonyms
Securin (PTTG1); Rat Securin (PTTG1) ELISA Kit; PTTG1; EAP1; HPTTG; MGC126883; MGC138276; PTTG; TUTR1; ESP1-associated protein 1; OTTHUMP00000160845; pituitary tumor-transforming protein 1; securin; tumor-transforming protein 1; PTTG1 elisa kit
Reactivity
Rat
Specificity
Rat Securin (PTTG1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTTG1. No significant cross-reactivity or interference between Rat PTTG1 and analogues was observed.
Sequence Length
199
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Securin (PTTG1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Securin (PTTG1) Standard Curve.)
Related Product Information for PTTG1 elisa kit
Description: This Rat Securin (PTTG1) ELISA Kit employs a two-site sandwich ELISA to quantitate PTTG1 in samples. An antibody specific for PTTG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTTG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTTG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTTG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Securin is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
Background: Securin is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
securin
NCBI Official Synonym Full Names
PTTG1 regulator of sister chromatid separation, securin
NCBI Official Symbol
Pttg1
NCBI Official Synonym Symbols
Pttg
NCBI Protein Information
securin
UniProt Protein Name
Securin
UniProt Gene Name
Pttg1
UniProt Synonym Gene Names
Pttg
Uniprot Description
Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of p53/TP53. The negative regulation of p53/TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation ().
Research Articles on PTTG1
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Product Notes
The Rat PTTG1 pttg1 (Catalog #AAA9712011) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712011 ELISA Kit recognizes Rat PTTG1. It is sometimes possible for the material contained within the vial of "Securin (PTTG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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