Human CD276 antigen ELISA Kit | CD276 elisa kit

Human CD276 antigen ELISA Kit

Cat. Num. AAA765657

• Gene Names: CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• Reactivity: Human
• Synonyms: CD276 antigen; Human CD276 antigen ELISA Kit; CD276/B7-H3/B7H3/B7 homolog 3/CD276 antigen/CD276 molecule/Costimulatory molecule; CD276 elisa kit

• Reactivity: Human
• Sequence Length: 534

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for CD276 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CD276 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CD276 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CD276 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CD276 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 67188443
• NCBI GeneID: 80381
• NCBI Accession #: NP_001019907.1
• NCBI GenBank Nucleotide #: NM_001024736.1
• Molecular Weight: 57,165 Da
• NCBI Official Full Name: CD276 antigen isoform a
• NCBI Official Synonym Full Names: CD276 molecule
• NCBI Official Symbol: CD276
• NCBI Official Synonym Symbols: B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
• NCBI Protein Information: CD276 antigen
• UniProt Protein Name: CD276 antigen
• Protein Family: CD276 antigen
• UniProt Gene Name: CD276
• UniProt Synonym Gene Names: B7H3; B7-H3
• UniProt Entry Name: CD276_HUMAN

NCBI Description

The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: external side of plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: cell proliferation; immune response; negative regulation of inflammatory response; negative regulation of interferon-gamma biosynthetic process; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell proliferation; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of T cell proliferation; regulation of immune response; T cell activation