Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regulator of G-protein signaling 6 (RGS6) Standard Curve.)
Rat Regulator Of G-Protein Signaling 6 (RGS6) ELISA Kit | RGS6 elisa kit
Rat Regulator Of G-Protein Signaling 6 (RGS6) ELISA Kit
Reactivity
Rat
Synonyms
Regulator Of G-Protein Signaling 6 (RGS6); Rat Regulator Of G-Protein Signaling 6 (RGS6) ELISA Kit; RGS6; DKFZp313G1241; FLJ43552; GAP; MGC142132; G protein signaling 6 regulator; GTPase activating protein; H_DJ0283M22.1; H_DJ1108A12.1; WUGSC:H_DJ0283M22.1; WUGSC:H_DJ1108A12.1; regulator of G protein signalin; RGS6 elisa kit
Reactivity
Rat
Specificity
Rat Regulator of G-protein signaling 6 (RGS6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RGS6. No significant cross-reactivity or interference between Rat RGS6 and analogues was observed.
Sequence Length
66
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regulator of G-protein signaling 6 (RGS6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Regulator of G-protein signaling 6 (RGS6) Standard Curve.)
Related Product Information for RGS6 elisa kit
Description: This Rat Regulator of G-protein signaling 6 (RGS6) ELISA Kit employs a two-site sandwich ELISA to quantitate RGS6 in samples. An antibody specific for RGS6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RGS6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGS6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGS6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Members of the RGS (regulator of G protein signaling) family have been shown to modulate the functioning of G proteins by activating the intrinsic GTPase activity of the alpha (guanine nucleotide-binding) subunits. RGS6, The predicted 472-amino acid protein has a calculated molecular mass of 54.4 kD. It contains an N-terminal dishevelled/egl10/pleckstrin (DEP) domain, a central G protein gamma subunit (GNG2)-like (GGL) domain, and an RGS domain near the C terminus. The variants encode proteins with long or short N-terminal domains, complete or incomplete GGL domains, 7 distinct C-terminal domains, and a common internal domain that includes the RGS domain.
Background: Members of the RGS (regulator of G protein signaling) family have been shown to modulate the functioning of G proteins by activating the intrinsic GTPase activity of the alpha (guanine nucleotide-binding) subunits. RGS6, The predicted 472-amino acid protein has a calculated molecular mass of 54.4 kD. It contains an N-terminal dishevelled/egl10/pleckstrin (DEP) domain, a central G protein gamma subunit (GNG2)-like (GGL) domain, and an RGS domain near the C terminus. The variants encode proteins with long or short N-terminal domains, complete or incomplete GGL domains, 7 distinct C-terminal domains, and a common internal domain that includes the RGS domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Regulator of G-protein signaling 6
NCBI Official Synonym Full Names
regulator of G-protein signaling 6
NCBI Official Symbol
Rgs6
NCBI Protein Information
regulator of G-protein signaling 6
UniProt Protein Name
Regulator of G-protein signaling 6
Protein Family
UniProt Gene Name
Rgs6
UniProt Synonym Gene Names
RGS6
NCBI Description
activates G-protein alpha GTPase activity; may play a role in negative regulation of G-protein signaling; human homolog may induce neuronal differentiation [RGD, Feb 2006]
Uniprot Description
Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form. The RGS6/GNB5 dimer enhances GNAO1 GTPase activity.
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Product Notes
The Rat RGS6 rgs6 (Catalog #AAA9711922) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711922 ELISA Kit recognizes Rat RGS6. It is sometimes possible for the material contained within the vial of "Regulator Of G-Protein Signaling 6 (RGS6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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