Typical Testing Data/Standard Curve (for reference only)
Rat Pyruvate carboxylase, mitochondrial, PC ELISA Kit | PC elisa kit
Rat Pyruvate carboxylase, mitochondrial, PC ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat PC antibody. PC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PC Antibody is added and binds to PC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated PC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat PC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
biotin-containing enzyme that catalyses the carboxylation of pyruvate to form oxaloacetate [RGD, Feb 2006]
Uniprot Description
PC: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate. Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency). PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Protein type: Mitochondrial; Carbohydrate Metabolism - citrate (TCA) cycle; Ligase; EC 6.4.1.1; Carbohydrate Metabolism - pyruvate
Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; mitochondrial inner membrane
Molecular Function: pyruvate carboxylase activity; DNA binding; carboxylic acid binding; metal ion binding; biotin carboxylase activity; biotin binding; ATP binding
Biological Process: oxaloacetate metabolic process; viral RNA genome packaging; lipid metabolic process; pyruvate metabolic process; gluconeogenesis
Research Articles on PC
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Product Notes
The Rat PC pc (Catalog #AAA1605289) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1605289 ELISA Kit recognizes Rat PC. It is sometimes possible for the material contained within the vial of "Pyruvate carboxylase, mitochondrial, PC, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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