Rat PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit | PYCARD elisa kit

Rat PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit

Cat. Num. AAA2098131

• Gene Names: PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
• Reactivity: Rat
• Synonyms: PYD And CARD Domain Containing Protein (PYCARD); Rat PYD And CARD Domain Containing Protein (PYCARD) ELISA Kit; ASC; CARD5; TMS1; Apoptosis-associated speck-like protein containing a CARD; Caspase recruitment domain-containing protein 5; Target of methylation-induced silencing 1; PYCARD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of PYD And CARD Domain Containing Protein (PYCARD).; No significant cross-reactivity or interference between PYD And CARD Domain Containing Protein (PYCARD) and analogues was observed.
• Sequence Length: 135

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.065ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PYD And CARD Domain Containing Protein (PYCARD) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PYD And CARD Domain Containing Protein (PYCARD) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PYCARD elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PYD And CARD Domain Containing Protein (PYCARD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PYD And CARD Domain Containing Protein (PYCARD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PYD And CARD Domain Containing Protein (PYCARD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PYD And CARD Domain Containing Protein (PYCARD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PYCARD elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 13325316
• NCBI GeneID: 29108
• Molecular Weight: 15,030 Da
• NCBI Official Full Name: PYD and CARD domain containing
• NCBI Official Synonym Full Names: PYD and CARD domain containing
• NCBI Official Symbol: PYCARD
• NCBI Official Synonym Symbols: ASC; TMS; TMS1; CARD5; TMS-1
• NCBI Protein Information: apoptosis-associated speck-like protein containing a CARD
• UniProt Protein Name: Apoptosis-associated speck-like protein containing a CARD
• Protein Family: Apoptosis-associated speck-like protein
• UniProt Gene Name: PYCARD
• UniProt Synonym Gene Names: ASC; CARD5; TMS1; hASC

NCBI Description

This gene encodes an adaptor protein that is composed of two protein-protein interaction domains: a N-terminal PYRIN-PAAD-DAPIN domain (PYD) and a C-terminal caspase-recruitment domain (CARD). The PYD and CARD domains are members of the six-helix bundle death domain-fold superfamily that mediates assembly of large signaling complexes in the inflammatory and apoptotic signaling pathways via the activation of caspase. In normal cells, this protein is localized to the cytoplasm; however, in cells undergoing apoptosis, it forms ball-like aggregates near the nuclear periphery. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PYCARD: Promotes caspase-mediated apoptosis. This proapoptotic activity is mediated predominantly through the activation of caspase-9. May be a component of the inflammasome, a protein complex which also includes NALP2, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Forms complexes with other DAPIN domain-containing proteins. Interacts with CIAS1/PYPAF1 and PYDC1. Widely expressed at low levels. Detected in peripheral blood leukocytes, lung, small intestine, spleen, thymus, colon and at lower levels in placenta, liver and kidney. Very low expression in skeletal muscle, heart and brain. Detected in the leukemia cell lines HL-60 and U-937, but not in Jurkat T- cell lymphoma and Daudi Burkitt's lymphoma. Detected in the melanoma cell line WM35, but not in WM793. Not detected in HeLa cervical carcinoma cells and MOLT-4 lymphocytic leukemia cells. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Tumor suppressor

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell soma; cytoplasm; cytosol; endoplasmic reticulum; extracellular region; IkappaB kinase complex; mitochondrion; NLRP1 inflammasome complex; NLRP3 inflammasome complex; nucleolus; nucleus; secretory granule lumen

Molecular Function: caspase activator activity; enzyme binding; identical protein binding; interleukin-6 receptor binding; ion channel binding; myosin I binding; protease binding; protein binding; protein homodimerization activity; Pyrin domain binding; tropomyosin binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of innate immune response; apoptosis; cellular response to interleukin-1; cellular response to tumor necrosis factor; defense response to Gram-negative bacterium; defense response to virus; inflammatory response; innate immune response; interleukin-1 beta production; intrinsic apoptotic signaling pathway by p53 class mediator; intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; macropinocytosis; myeloid dendritic cell activation; myeloid dendritic cell activation involved in immune response; negative regulation of I-kappaB kinase/NF-kappaB signaling; negative regulation of interferon-beta production; negative regulation of NF-kappaB transcription factor activity; negative regulation of protein serine/threonine kinase activity; neutrophil degranulation; positive regulation of actin filament polymerization; positive regulation of activated T cell proliferation; positive regulation of adaptive immune response; positive regulation of antigen processing and presentation of peptide antigen via MHC class II; positive regulation of apoptosis; positive regulation of chemokine secretion; positive regulation of cysteine-type endopeptidase activity; positive regulation of cysteine-type endopeptidase activity involved in apoptotic process; positive regulation of defense response to virus by host; positive regulation of ERK1 and ERK2 cascade; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of interferon-gamma production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-10 secretion; positive regulation of interleukin-6 production; positive regulation of interleukin-6 secretion; positive regulation of interleukin-8 secretion; positive regulation of JNK cascade; positive regulation of NF-kappaB transcription factor activity; positive regulation of phagocytosis; positive regulation of release of cytochrome c from mitochondria; positive regulation of T cell activation; positive regulation of T cell migration; positive regulation of transcription factor activity; positive regulation of tumor necrosis factor production; regulation of autophagy; regulation of GTPase activity; regulation of inflammatory response; regulation of intrinsic apoptotic signaling pathway; regulation of protein stability; regulation of tumor necrosis factor-mediated signaling pathway; signal transduction; tumor necrosis factor-mediated signaling pathway