Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) Standard Curve.)
Rat Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6) ELISA Kit | PCSK6 elisa kit
Rat Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6) ELISA Kit
Gene Names
Pcsk6; Spc4; Pace4
Reactivity
Rat
Synonyms
Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6); Rat Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6) ELISA Kit; PCSK6; PACE4; SPC4; paired basic amino acid cleaving system 4; subtilisin-like proprotein convertase 4; subtilisin-like protease; subtilisin/kexin-like protease PACE4; PCSK6 elisa kit
Reactivity
Rat
Specificity
Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PCSK6. No significant cross-reactivity or interference between Rat PCSK6 and analogues was observed.
Sequence Length
937
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) Standard Curve.)
Related Product Information for PCSK6 elisa kit
Description: This Rat Proprotein convertase subtilisin/kexin type 6 (PCSK6) ELISA Kit employs a two-site sandwich ELISA to quantitate PCSK6 in samples. An antibody specific for PCSK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCSK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proprotein convertase subtilisin/kexin type 6 is an enzyme that belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Each PACE4 isoform exhibits a unique restricted distribution. PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. PACE4A-II is at least expressed in placenta. PACE4B was only found in the embryonic kidney cell line from which it was isolated. PACE4C and PACE4D are expressed in placenta.
Background: Proprotein convertase subtilisin/kexin type 6 is an enzyme that belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. Each PACE4 isoform exhibits a unique restricted distribution. PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. PACE4A-II is at least expressed in placenta. PACE4B was only found in the embryonic kidney cell line from which it was isolated. PACE4C and PACE4D are expressed in placenta.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
proprotein convertase subtilisin/kexin type 6
NCBI Official Synonym Full Names
proprotein convertase subtilisin/kexin type 6
NCBI Official Symbol
Pcsk6
NCBI Official Synonym Symbols
Spc4; Pace4
NCBI Protein Information
proprotein convertase subtilisin/kexin type 6
UniProt Protein Name
Proprotein convertase subtilisin/kexin type 6
Protein Family
UniProt Gene Name
Pcsk6
UniProt Synonym Gene Names
Pace4; SPC4
UniProt Entry Name
PCSK6_RAT
NCBI Description
may play a role in neuroendocrine peptide processing [RGD, Feb 2006]
Research Articles on PCSK6
Similar Products
Product Notes
The Rat PCSK6 pcsk6 (Catalog #AAA9712141) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712141 ELISA Kit recognizes Rat PCSK6. It is sometimes possible for the material contained within the vial of "Proprotein Convertase Subtilisin/Kexin Type 6 (PCSK6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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