Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proenkephalin-B (PDYN) Standard Curve.)
Rat Proenkephalin-B (PDYN) ELISA Kit | PDYN elisa kit
Rat Proenkephalin-B (PDYN) ELISA Kit
Reactivity
Rat
Synonyms
Proenkephalin-B (PDYN); Rat Proenkephalin-B (PDYN) ELISA Kit; PDYN; MGC26418; PENKB; beta-neoendorphin; beta-neoendorphin-dynorphin; dynorphin; leu-enkephalin; leumorphin; preprodynorphin; preproenkephalin B; rimorphin; PDYN elisa kit
Reactivity
Rat
Specificity
Rat Proenkephalin-B (PDYN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDYN. No significant cross-reactivity or interference between Rat PDYN and analogues was observed.
Sequence Length
248
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proenkephalin-B (PDYN) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Proenkephalin-B (PDYN) Standard Curve.)
Related Product Information for PDYN elisa kit
Description: This Rat Proenkephalin-B (PDYN) ELISA Kit employs a two-site sandwich ELISA to quantitate PDYN in samples. An antibody specific for PDYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PDYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Prodynorphin is a opioid polypeptide hormone involved with chemical signal transduction and cell communication. The gene for prodynorphin is expressed in the endometrium and the striatum, and its gene map locus is 20pter-p12. Prodynorphin is a basic building block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds, and which are also critical in learning and memory. The prodynorphin enzyme is identical in humans and chimps, but the regulatory promoter sequences have been shown to exhibit marked differences.The gene is thought to influence perception, as well as susceptibility to drug dependence, and is expressed more readily in human beings than in other primates.
Background: Prodynorphin is a opioid polypeptide hormone involved with chemical signal transduction and cell communication. The gene for prodynorphin is expressed in the endometrium and the striatum, and its gene map locus is 20pter-p12. Prodynorphin is a basic building block of endorphins, the chemical messengers in the brain that appear most heavily involved in the anticipation and experience of pain and the formation of deep emotional bonds, and which are also critical in learning and memory. The prodynorphin enzyme is identical in humans and chimps, but the regulatory promoter sequences have been shown to exhibit marked differences.The gene is thought to influence perception, as well as susceptibility to drug dependence, and is expressed more readily in human beings than in other primates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Proenkephalin-B
NCBI Official Synonym Full Names
prodynorphin
NCBI Official Symbol
Pdyn
NCBI Protein Information
proenkephalin-B
UniProt Protein Name
Proenkephalin-B
Protein Family
UniProt Gene Name
Pdyn
UniProt Synonym Gene Names
Big Dyn; Dyn-A17; Dynorphin A; Dyn-B
UniProt Entry Name
PDYN_RAT
NCBI Description
expression in hippocampus increases in response to traumatic brain injury; may play a role in regulatio of neurotransmitter release [RGD, Feb 2006]
Uniprot Description
Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress ().
Research Articles on PDYN
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Product Notes
The Rat PDYN pdyn (Catalog #AAA9712132) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712132 ELISA Kit recognizes Rat PDYN. It is sometimes possible for the material contained within the vial of "Proenkephalin-B (PDYN), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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