Rat Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit | PIICP elisa kit

Rat Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit

Cat. Num. AAA2097778

• Gene Names: Col2a1; CG2A1A; COLLII
• Reactivity: Rat
• Synonyms: Procollagen II C-Terminal Propeptide (PIICP); Rat Procollagen II C-Terminal Propeptide (PIICP) ELISA Kit; P2CP; C-Propeptide Of Type II Procollagen; Procollagen II Carboxy Terminal Propeptide; PIICP elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Procollagen II C-Terminal Propeptide (PIICP).; No significant cross-reactivity or interference between Procollagen II C-Terminal Propeptide (PIICP) and analogues was observed.
• Sequence Length: 1419

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 3.70-300ng/mL
• Sensitivity: < 1.35ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Procollagen II C-Terminal Propeptide (PIICP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PIICP elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Procollagen II C-Terminal Propeptide (PIICP) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Procollagen II C-Terminal Propeptide (PIICP) and unlabeled Procollagen II C-Terminal Propeptide (PIICP) (Standards or samples) with the pre-coated antibody specific to Procollagen II C-Terminal Propeptide (PIICP). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Procollagen II C-Terminal Propeptide (PIICP) in the sample.

Product Categories/Family for PIICP elisa kit

Infection immunity; Bone metabolism; Rheumatology

NCBI and Uniprot Product Information

• NCBI GI #: 6978677
• NCBI GeneID: 25412
• NCBI Accession #: NP_037061.1
• NCBI GenBank Nucleotide #: NM_012929.1
• UniProt Accession #: P05539; Q63123; Q63565; Q78DY3
• Molecular Weight: 134,570 Da
• NCBI Official Full Name: collagen alpha-1(II) chain
• NCBI Official Synonym Full Names: collagen type II alpha 1 chain
• NCBI Official Symbol: Col2a1
• NCBI Official Synonym Symbols: CG2A1A; COLLII
• NCBI Protein Information: collagen alpha-1(II) chain
• UniProt Protein Name: Collagen alpha-1(II) chain
• UniProt Gene Name: Col2a1

NCBI Description

structural component of the cartilage extracellular matrix [RGD, Feb 2006]

Uniprot Description

Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces.