Rabbit anti-Mouse COX5A Polyclonal Antibody | anti-COX5A antibody

COX5A, NT (COX5A, Cytochrome c oxidase subunit 5A, mitochondrial, Cytochrome c oxidase polypeptide Va) (AP)

Cat. Num. AAA6288087

• Gene Names: COX5A; VA; COX; COX-VA
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: COX5A; Polyclonal Antibody; NT (COX5A; Cytochrome c oxidase subunit 5A; mitochondrial; Cytochrome c oxidase polypeptide Va) (AP); Cytochrome c oxidase polypeptide Va; anti-COX5A antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-COX5A antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: COX5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-55 amino acids from the N-terminal region of human COX5A.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(COX5A Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the COX5A antibody detected the COX5A protein (arrow).)

Related Product Information for anti-COX5A antibody

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial-and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq].

Product Categories/Family for anti-COX5A antibody

Antibodies; Enzymes; Cytochrome

References

Chen, Z.X., et al. Cell Death Differ. 17(3):408-420(2010). Fornuskova, D., et al. Biochem. J. 428(3):363-374(2010). Uddin, M., et al. BMC Evol. Biol. 8, 8 (2008): Williams, S.L., et al. J. Biol. Chem. 279(9):7462-7469(2004). Hofmann, S., et al. Cytogenet. Cell Genet. 83 (3-4), 226-227 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 190885499
• NCBI GeneID: 9377
• NCBI Accession #: NP_004246.2
• NCBI GenBank Nucleotide #: NM_004255.3
• UniProt Accession #: P20674; P30045; Q8TB65
• Molecular Weight: 16,762 Da
• NCBI Official Full Name: cytochrome c oxidase subunit 5A, mitochondrial
• NCBI Official Synonym Full Names: cytochrome c oxidase subunit Va
• NCBI Official Symbol: COX5A
• NCBI Official Synonym Symbols: VA; COX; COX-VA
• NCBI Protein Information: cytochrome c oxidase subunit 5A, mitochondrial; cytochrome c oxidase polypeptide Va; mitochondrial cytochrome c oxidase subunit Va; cytochrome c oxidase polypeptide, mitochondrial
• UniProt Protein Name: Cytochrome c oxidase subunit 5A, mitochondrial
• Protein Family: Cytochrome c oxidase
• UniProt Gene Name: COX5A
• UniProt Entry Name: COX5A_HUMAN

NCBI Description

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer of proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit Va of the human mitochondrial respiratory chain enzyme. A pseudogene COX5AP1 has been found in chromosome 14q22. [provided by RefSeq, Jul 2008]

Uniprot Description

COX5A: This is the heme A-containing chain of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. Belongs to the cytochrome c oxidase subunit 5A family.

Protein type: Energy Metabolism - oxidative phosphorylation; EC 1.9.3.1; Oxidoreductase; Mitochondrial

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: mitochondrial inner membrane

Molecular Function: cytochrome-c oxidase activity; electron carrier activity; metal ion binding

Biological Process: cellular metabolic process; transmembrane transport