Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Probable proline dehydrogenase 2 (PRODH2) Standard Curve.)
Rat Probable Proline Dehydrogenase 2 (PRODH2) ELISA Kit | PRODH2 elisa kit
Rat Probable Proline Dehydrogenase 2 (PRODH2) ELISA Kit
Gene Names
Prodh2; HYPDH
Reactivity
Rat
Synonyms
Probable Proline Dehydrogenase 2 (PRODH2); Rat Probable Proline Dehydrogenase 2 (PRODH2) ELISA Kit; PRODH2; HSPOX1; kidney and liver proline oxidase 1; proline dehydrogenase 2; PRODH2 elisa kit
Reactivity
Rat
Specificity
Rat Probable proline dehydrogenase 2 (PRODH2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRODH2. No significant cross-reactivity or interference between Rat PRODH2 and analogues was observed.
Sequence Length
456
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Probable proline dehydrogenase 2 (PRODH2) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Probable proline dehydrogenase 2 (PRODH2) Standard Curve.)
Related Product Information for PRODH2 elisa kit
Description: This Rat Probable proline dehydrogenase 2 (PRODH2) ELISA Kit employs a two-site sandwich ELISA to quantitate PRODH2 in samples. An antibody specific for PRODH2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRODH2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRODH2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRODH2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by PRODH2 gene catalyzes the first step in the catabolism of trans-4-hydroxy-L-proline, an amino acid derivative obtained through food intake and collagen turnover. One of the downstream products of this catabolism is glyoxylate, which in people with disorders of glyoxalate metabolism can lead to an increase in oxalate levels and the formation of calcium-oxalate kidney stones. Therefore, this gene may serve as a therapeutic target against primary hyperoxalurias (PH). This gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme that catalyzes the first step in proline catabolism. [provided by RefSeq, Jan 2017]
Background: The protein encoded by PRODH2 gene catalyzes the first step in the catabolism of trans-4-hydroxy-L-proline, an amino acid derivative obtained through food intake and collagen turnover. One of the downstream products of this catabolism is glyoxylate, which in people with disorders of glyoxalate metabolism can lead to an increase in oxalate levels and the formation of calcium-oxalate kidney stones. Therefore, this gene may serve as a therapeutic target against primary hyperoxalurias (PH). This gene is similar to proline dehydrogenase (oxidase) 1, a mitochondrial enzyme that catalyzes the first step in proline catabolism. [provided by RefSeq, Jan 2017]
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Hydroxyproline dehydrogenase
NCBI Official Synonym Full Names
proline dehydrogenase 2
NCBI Official Symbol
Prodh2
NCBI Official Synonym Symbols
HYPDH
NCBI Protein Information
hydroxyproline dehydrogenase
UniProt Protein Name
Probable proline dehydrogenase 2
Protein Family
UniProt Gene Name
Prodh2
UniProt Entry Name
PROD2_RAT
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Product Notes
The Rat PRODH2 prodh2 (Catalog #AAA9712051) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712051 ELISA Kit recognizes Rat PRODH2. It is sometimes possible for the material contained within the vial of "Probable Proline Dehydrogenase 2 (PRODH2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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