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Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Probable histone acetyltransferase MYST1 (MYST1) Standard Curve.)

Rat Probable Histone Acetyltransferase MYST1 (MYST1) ELISA Kit | MYST1 elisa kit

Rat Probable Histone Acetyltransferase MYST1 (MYST1) ELISA Kit

Gene Names
Kat8; Myst1
Reactivity
Rat
Synonyms
Probable Histone Acetyltransferase MYST1 (MYST1); Rat Probable Histone Acetyltransferase MYST1 (MYST1) ELISA Kit; MYST1; FLJ14040; KAT8; MOF; hMOF; MOZ; YBF2/SAS3; SAS2 and TIP60 protein 1; histone acetyltransferase MYST1; ortholog of Drosophila males absent on the first (MOF); MYST1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
Rat Probable histone acetyltransferase MYST1 (MYST1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MYST1. No significant cross-reactivity or interference between Rat MYST1 and analogues was observed.
Sequence Length
458
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Probable histone acetyltransferase MYST1 (MYST1) Standard Curve.)

Typical Testing Data/Standard Curve (for reference only) (Fig.1. Rat Probable histone acetyltransferase MYST1 (MYST1) Standard Curve.)
Related Product Information for MYST1 elisa kit
Description: This Rat Probable histone acetyltransferase MYST1 (MYST1) ELISA Kit employs a two-site sandwich ELISA to quantitate MYST1 in samples. An antibody specific for MYST1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MYST1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYST1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYST1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: KAT8 encodes a member of the MYST histone acetylase protein family. The encoded protein has a characteristic MYST domain containing an acetyl-CoA-binding site, a chromodomain typical of proteins which bind histones, and a C2HC-type zinc finger. Multiple transcript variants encoding different isoforms have been found for KAT8.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
histone acetyltransferase KAT8
NCBI Official Synonym Full Names
lysine acetyltransferase 8
NCBI Official Symbol
Kat8
NCBI Official Synonym Symbols
Myst1
NCBI Protein Information
histone acetyltransferase KAT8
UniProt Protein Name
Histone acetyltransferase KAT8
UniProt Gene Name
Kat8
UniProt Synonym Gene Names
Myst1; MYST-1
UniProt Entry Name
KAT8_RAT

Uniprot Description

MYST1: Histone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Belongs to the MYST (SAS/MOZ) family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.48; Acetyltransferase

Cellular Component: histone acetyltransferase complex; kinetochore; nuclear membrane; nucleoplasm; nucleus

Molecular Function: acetyltransferase activity; enzyme binding; histone acetyltransferase activity; histone acetyltransferase activity (H4-K16 specific); metal ion binding; methylated histone residue binding; transcription factor binding

Biological Process: histone acetylation; myeloid cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of autophagy; transcription, DNA-dependent

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Product Notes

The Rat MYST1 kat8 (Catalog #AAA9712182) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712182 ELISA Kit recognizes Rat MYST1. It is sometimes possible for the material contained within the vial of "Probable Histone Acetyltransferase MYST1 (MYST1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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