Western Blot (WB)
(Western blot analysis of human breast cancer tissue lysate (35ug protein in RIPA buffer) probed with NLRX1 antibody (2ug/ml,MBS226218) and detected by chemiluminescence.)
Goat anti-Human NLRX1 Polyclonal Antibody | anti-NLRX1 antibody
Goat anti NLRX1
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Purified IgG - liquid
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Western Blot (WB)
(Western blot analysis of human breast cancer tissue lysate (35ug protein in RIPA buffer) probed with NLRX1 antibody (2ug/ml,MBS226218) and detected by chemiluminescence.)
Western Blot (WB)
(Western blot analysis of human breast cancer tissue lysate (35ug protein in RIPA buffer) probed with NLRX1 antibody (2ug/ml,MBS226218) and detected by chemiluminescence.)
NLRX1 is a member of the NLR family of pattern recognition receptors (PRRs), but is an atypical member due to its structure (Nagai-Singer et al. 2019). For example, it appears to be the only PRR family member with the ability to localize to mitochondria (Chu et al. 2019). However, NLRX1 appears to be ubituitously expressed in human cells, and appears to have different functions depending on cell type (Nagai-Singer et al. 2019). NLRX1 appears to be a negative regulator of immune signaling during microbial infection, cancer, and autoimmune disease (Kastelberg et al. 2020). Consistent with mitochondrial localization, NLRX1 regulates the function of mitochondria during apoptosis and injury in the presence of TNF-alpha (Singh et al. 2019).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the NLR family and localizes to the outer mitochondrial membrane. The encoded protein is a regulator of mitochondrial antivirus responses. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2013]
Uniprot Description
NLRX1: Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF- Kappa-B and type 1 interferon signaling pathways, but enhances NF- Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). Interacts with MAVS. Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HeLa, HEK293T, THP-1, HL-60, Raji, and Jurkat cell lines. Belongs to the NLRP family. 2 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: mitochondrial outer membrane
Molecular Function: protein binding; ATP binding
Biological Process: viral reproduction; negative regulation of innate immune response; negative regulation of inflammatory response; negative regulation of interleukin-6 production; innate immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interferon type I production; negative regulation of interferon-beta production
Research Articles on NLRX1
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Product Notes
The NLRX1 nlrx1 (Catalog #AAA226218) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti NLRX1 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NLRX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1ug/ml to 3ug/ml. Researchers should empirically determine the suitability of the NLRX1 nlrx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NLRX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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