Rat Presenilin 2 (PS2) ELISA Kit | PS2 elisa kit

Rat Presenilin 2 (PS2) ELISA Kit

Cat. Num. AAA2019598

• Gene Names: PSEN2; AD4; PS2; AD3L; STM2; CMD1V
• Reactivity: Rat
• Synonyms: Presenilin 2 (PS2); Rat Presenilin 2 (PS2) ELISA Kit; PSEN2; AD3L; AD4; PS2; STM2; AD3LP; AD5; Alzheimer Disease 4; PS2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Presenilin 2 (PS2).; No significant cross-reactivity or interference between Presenilin 2 (PS2) and analogues was observed.
• Sequence Length: 447

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Presenilin 2 (PS2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Presenilin 2 (PS2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PS2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Presenilin 2 (PS2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Presenilin 2 (PS2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Presenilin 2 (PS2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Presenilin 2 (PS2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for PS2 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 156105681
• NCBI GeneID: 5664
• NCBI Accession #: NP_036618.2
• NCBI GenBank Nucleotide #: NM_012486.2
• UniProt Accession #: P49810; Q96P32; A8K8D4; B1AP21
• Molecular Weight: 50,011 Da
• NCBI Official Full Name: presenilin-2 isoform 2
• NCBI Official Synonym Full Names: presenilin 2
• NCBI Official Symbol: PSEN2
• NCBI Official Synonym Symbols: AD4; PS2; AD3L; STM2; CMD1V
• NCBI Protein Information: presenilin-2
• UniProt Protein Name: Presenilin-2
• Protein Family: Inorganic pyrophosphatase
• UniProt Gene Name: PSEN2
• UniProt Synonym Gene Names: AD4; PS2; PSNL2; STM2; PS-2
• UniProt Entry Name: PSN2_HUMAN

NCBI Description

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

PSEN2: probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). An integral membrane protein of the Golgi and endoplasmic reticulum. Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. Two alternative splice isoforms have been identified.

Protein type: Protease; EC 3.4.23.-; Membrane protein, multi-pass; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 1q42.13

Cellular Component: Golgi apparatus; centrosome; cell surface; lysosomal membrane; integral to plasma membrane; nuclear inner membrane; cytosol; lipid raft; kinetochore; ciliary rootlet; cell soma; membrane; perinuclear region of cytoplasm; mitochondrial inner membrane; apical plasma membrane; dendritic shaft; neuromuscular junction; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cell cortex; Z disc; Golgi membrane; growth cone; axon; plasma membrane

Molecular Function: protein binding; endopeptidase activity; aspartic-type endopeptidase activity

Biological Process: positive regulation of catalytic activity; axon guidance; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; protein maturation; regulation of synaptic plasticity; positive regulation of coagulation; cell fate specification; T cell receptor signaling pathway; T cell activation during immune response; protein transport; amyloid precursor protein catabolic process; negative regulation of protein amino acid phosphorylation; hair follicle development; beta-amyloid metabolic process; calcium ion transport; forebrain development; ephrin receptor signaling pathway; brain morphogenesis; negative regulation of protein binding; dorsoventral neural tube patterning; cardiac muscle contraction; embryonic limb morphogenesis; somitogenesis; Notch signaling pathway; membrane protein intracellular domain proteolysis; thymus development; membrane protein ectodomain proteolysis; regulation of epidermal growth factor receptor activity; Notch receptor processing; memory; endoplasmic reticulum calcium ion homeostasis; myeloid leukocyte differentiation; response to hypoxia; hemopoietic progenitor cell differentiation; protein processing; negative regulation of protein complex assembly; alveolus development; negative regulation of apoptosis

Disease: Alzheimer Disease 4; Cardiomyopathy, Dilated, 1v