Rabbit anti-Human NEK3 Polyclonal Antibody | anti-NEK3 antibody

NEK3 (Serine/Threonine-Protein Kinase Nek3, Never In Mitosis A-Related Kinase 3, NimA-Related Protein Kinase 3, HSPK 36, MGC29949) (Biotin)

Cat. Num. AAA6386855

• Gene Names: NEK3; HSPK36
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NEK3; Polyclonal Antibody; NEK3 (Serine/Threonine-Protein Kinase Nek3; Never In Mitosis A-Related Kinase 3; NimA-Related Protein Kinase 3; HSPK 36; MGC29949) (Biotin); anti-NEK3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NEK3.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 506

• Applicable Applications for anti-NEK3 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human NEK3, aa1-506 (NP_002489.1).
• Immunogen Sequence: MDDYMVLRMIGEGSFGRALLVQHESSNQMFAMKEIRLPKSFSNTQNSRKEAVLLAKMKHPNIVAFKESFEAEGHLYIVMEYCDGGDLMQKIKQQKGKLFPEDMILNWFTQMCLGVNHIHKKRVLHRDIKSKNIFLTQNGKVKLGDFGSARLLSNPMAFACTYVGTPYYVPPEIWENLPYNNKSDIWSLGCILYELCTLKHPFQANSWKNLILKVCQGCISPLPSHYSYELQFLVKQMFKRNPSHRPSATTLLSRGIVARLVQKCLPPEIIMEYGEEVLEEIKNSKHNTPRKKTNPSRIRIALGNEASTVQEEEQDRKGSHTDLESINENLVESALRRVNREEKGNKSVHLRKASSPNLHRRQWEKNVPNTALTALENASILTSSLTAEDDRGGSVIKYSKNTTRKQWLKETPDTLLNILKNADLSLAFQTYTIYRPGSEGFLKGPLSEETEASDSVDGGHDSVILDPERLEPGLDEEDTDFEEEDDNPDWVSELKKRAGWQGLCDR
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of NEK3 expression in transfected 293T cell line by NEK3 polyclonal antibody. Lane 1: NEK3 transfected lysate (57.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-NEK3 antibody

In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized. [provided by RefSeq]

Product Categories/Family for anti-NEK3 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 23510391
• NCBI GeneID: 4752
• NCBI Accession #: NP_002489.1
• NCBI GenBank Nucleotide #: NM_002498.1
• UniProt Accession #: P51956
• NCBI Official Full Name: serine/threonine-protein kinase Nek3 isoform a
• NCBI Official Synonym Full Names: NIMA related kinase 3
• NCBI Official Symbol: NEK3
• NCBI Official Synonym Symbols: HSPK36
• NCBI Protein Information: serine/threonine-protein kinase Nek3
• UniProt Protein Name: Serine/threonine-protein kinase Nek3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK3
• UniProt Synonym Gene Names: NimA-related protein kinase 3

NCBI Description

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. Two functional alleles for this gene have been identified in humans. The reference genome assembly (GRCh38) represents a functional allele that is associated with the inclusion of an additional coding exon in protein-coding transcripts, compared to an alternate functional allele that lacks the exon. [provided by RefSeq, Sep 2019]

Uniprot Description

Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. Implicated in prolactin-mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.