Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasma protease C1 inhibitor (SERPING1) Standard Curve.)
Rat Plasma Protease C1 Inhibitor (SERPING1) ELISA Kit | SERPING1 elisa kit
Rat Plasma Protease C1 Inhibitor (SERPING1) ELISA Kit
Gene Names
SERPING1; C1Inh
Reactivity
Rat
Synonyms
Plasma Protease C1 Inhibitor (SERPING1); Rat Plasma Protease C1 Inhibitor (SERPING1) ELISA Kit; SERPING1; C1IN; C1INH; C1NH; HAE1; HAE2; complement component 1 inhibitor; plasma protease C1 inhibitor; serine/cysteine proteinase inhibitor clade G member 1; serpin peptidase inhibitor; clade G; member 1; SERPING1 elisa kit
Reactivity
Rat
Specificity
Rat Plasma protease C1 inhibitor (SERPING1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPING1. No significant cross-reactivity or interference between Rat SERPING1 and analogues was observed.
Sequence Length
18
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasma protease C1 inhibitor (SERPING1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Plasma protease C1 inhibitor (SERPING1) Standard Curve.)
Related Product Information for SERPING1 elisa kit
Description: This Rat Plasma protease C1 inhibitor (SERPING1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPING1 in samples. An antibody specific for SERPING1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPING1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPING1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPING1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: C1-INH is a critically important protein that controls activation of multiple plasma mediator pathways. This protein, a member of the serine protease inhibitor (serpin) group, originally was described as an inhibitor of C1. It binds stoichiometrically to the active sites on both C1r and C1s to form a complex C1-INH-C1r-C1s-C1-INH and thus inhibits activated C1. In addition, C1-INH has been reported to remove the intact C1qrs complex from an activating surface and to inhibit autoactivation of C1. C1-INH is a known inhibitor of kinin generating (kallikrein), fibrinolytic (plasmin), and contact activation (intrinsic) pathway of the coagulation cascade. Recently, it has been shown to be an inhibitor of the mannan-binding lectin pathway of complement activation, inhibiting mannan-binding lectin-associated serine proteases (MASPs) in that pathway.
Background: C1-INH is a critically important protein that controls activation of multiple plasma mediator pathways. This protein, a member of the serine protease inhibitor (serpin) group, originally was described as an inhibitor of C1. It binds stoichiometrically to the active sites on both C1r and C1s to form a complex C1-INH-C1r-C1s-C1-INH and thus inhibits activated C1. In addition, C1-INH has been reported to remove the intact C1qrs complex from an activating surface and to inhibit autoactivation of C1. C1-INH is a known inhibitor of kinin generating (kallikrein), fibrinolytic (plasmin), and contact activation (intrinsic) pathway of the coagulation cascade. Recently, it has been shown to be an inhibitor of the mannan-binding lectin pathway of complement activation, inhibiting mannan-binding lectin-associated serine proteases (MASPs) in that pathway.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Plasma protease C1 inhibitor
NCBI Official Symbol
SERPING1
NCBI Official Synonym Symbols
C1Inh
NCBI Protein Information
plasma protease C1 inhibitor
UniProt Protein Name
Plasma protease C1 inhibitor
Protein Family
UniProt Gene Name
SERPING1
UniProt Synonym Gene Names
C1Inh
Uniprot Description
Activation of the C1 complex is under control of the C1-inhibitor. It forms a proteolytically inactive stoichiometric complex with the C1r or C1s proteases. May play a potentially crucial role in regulating important physiological pathways including complement activation, blood coagulation, fibrinolysis and the generation of kinins. Very efficient inhibitor of FXIIa. Inhibits chymotrypsin and kallikrein.
Similar Products
Product Notes
The Rat SERPING1 serping1 (Catalog #AAA9711890) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711890 ELISA Kit recognizes Rat SERPING1. It is sometimes possible for the material contained within the vial of "Plasma Protease C1 Inhibitor (SERPING1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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