Typical Testing Data/Standard Curve (for reference only)
Rat Plasma membrane calcium-transporting ATPase 1 (ATP2B1) ELISA Kit | ATP2B1 elisa kit
Rat Plasma membrane calcium-transporting ATPase 1 (ATP2B1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: ATP2B1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATP2B1 antibody and an ATP2B1-HRP conjugate. The assay sample and buffer are incubated together with ATP2B1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATP2B1 concentration since ATP2B1 from samples and ATP2B1-HRP conjugate compete for the anti-ATP2B1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATP2B1 from the sample, fewer sites are left to bind ATP2B1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATP2B1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
PMCA1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. 6 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Transporter; Membrane protein, multi-pass; EC 3.6.3.8; Hydrolase; Transporter, ion channel
Chromosomal Location of Human Ortholog: 12q21.3
Cellular Component: membrane; integral to plasma membrane; plasma membrane; nucleus
Molecular Function: calmodulin binding; protein binding; calcium-transporting ATPase activity; metal ion binding; ATP binding
Biological Process: transport; metabolic process; blood coagulation; transmembrane transport
Research Articles on ATP2B1
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Product Notes
The Rat ATP2B1 atp2b1 (Catalog #AAA7246348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7246348 ELISA Kit recognizes Rat ATP2B1. It is sometimes possible for the material contained within the vial of "Plasma membrane calcium-transporting ATPase 1 (ATP2B1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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