Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipid scramblase 1 (PLSCR1) Standard Curve.)
Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit | PLSCR1 elisa kit
Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit
Reactivity
Rat
Synonyms
Phospholipid scramblase 1 (PLSCR1); Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit; PLSCR1; MMTRA1B; PLSCR1 elisa kit
Reactivity
Rat
Specificity
Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLSCR1. No significant cross-reactivity or interference between Rat PLSCR1 and analogues was observed.
Sequence Length
335
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipid scramblase 1 (PLSCR1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipid scramblase 1 (PLSCR1) Standard Curve.)
Related Product Information for PLSCR1 elisa kit
Description: This Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit employs a two-site sandwich ELISA to quantitate PLSCR1 in samples. An antibody specific for PLSCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLSCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLSCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLSCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Scramblases are members of the general family of transmembrane lipid transporters known as flippases. The inner-leaflet facing the inside of the cell contains negatively-charged amino-phospholipids and phosphatidylethanolamine. The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin. Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner-leaflet to the outer-leaflet, and vice versa.Wiedmer et al. (2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb. The first exon is untranslated. By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.
Background: Scramblases are members of the general family of transmembrane lipid transporters known as flippases. The inner-leaflet facing the inside of the cell contains negatively-charged amino-phospholipids and phosphatidylethanolamine. The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin. Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner-leaflet to the outer-leaflet, and vice versa.Wiedmer et al. (2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb. The first exon is untranslated. By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phospholipid scramblase 1
NCBI Official Synonym Full Names
phospholipid scramblase 1
NCBI Official Symbol
Plscr1
NCBI Protein Information
phospholipid scramblase 1
UniProt Protein Name
Phospholipid scramblase 1
Protein Family
UniProt Gene Name
Plscr1
UniProt Synonym Gene Names
PL scramblase 1
UniProt Entry Name
PLS1_RAT
NCBI Description
may act as a downstream mediator of immune response in IgE receptor signaling; may transport phospholipids in the plasma membrane [RGD, Feb 2006]
Research Articles on PLSCR1
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Product Notes
The Rat PLSCR1 plscr1 (Catalog #AAA9712106) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712106 ELISA Kit recognizes Rat PLSCR1. It is sometimes possible for the material contained within the vial of "Phospholipid scramblase 1 (PLSCR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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