Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipase A1 member A (PLA1A) Standard Curve.)
Rat Phospholipase A1 Member A (PLA1A) ELISA Kit | PLA1A elisa kit
Rat Phospholipase A1 Member A (PLA1A) ELISA Kit
Gene Names
Pla1a; Pspla1; Ps-pla1
Reactivity
Rat
Synonyms
Phospholipase A1 Member A (PLA1A); Rat Phospholipase A1 Member A (PLA1A) ELISA Kit; PLA1A; PS-PLA1; PSPLA1; phosphatidylserine-specific phospholipase A1alpha; PLA1A elisa kit
Reactivity
Rat
Specificity
Rat Phospholipase A1 member A (PLA1A) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLA1A. No significant cross-reactivity or interference between Rat PLA1A and analogues was observed.
Sequence Length
456
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipase A1 member A (PLA1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Phospholipase A1 member A (PLA1A) Standard Curve.)
Related Product Information for PLA1A elisa kit
Description: This Rat Phospholipase A1 member A (PLA1A) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA1A in samples. An antibody specific for PLA1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Phospholipase A1 is a phospholipase enzyme which removes the 1-acyl. Phospholipase A1 is a component of snake venom, but it also serves in normal metabolism to produce fatty acids from phospholipids. In melanocytic cells the Phospholipase A1 gene may be regulated by MITF. Phosphatidylserine-specific phospholipase A1-alpha (PLA1A) acts specifically on phosphatidylserine (PS) and 1-acyl-2-lysophosphatidylserine (lyso-PS) to hydrolyze fatty acids at the sn-1 position of these phospholipids.Although PLA1A belongs to the phospholipase gene family, the predicted amino acid sequence of the rat gene product demonstrated greater homology with mammalian triglyceride lipases than with phospholipases.
Background: Phospholipase A1 is a phospholipase enzyme which removes the 1-acyl. Phospholipase A1 is a component of snake venom, but it also serves in normal metabolism to produce fatty acids from phospholipids. In melanocytic cells the Phospholipase A1 gene may be regulated by MITF. Phosphatidylserine-specific phospholipase A1-alpha (PLA1A) acts specifically on phosphatidylserine (PS) and 1-acyl-2-lysophosphatidylserine (lyso-PS) to hydrolyze fatty acids at the sn-1 position of these phospholipids.Although PLA1A belongs to the phospholipase gene family, the predicted amino acid sequence of the rat gene product demonstrated greater homology with mammalian triglyceride lipases than with phospholipases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
phospholipase A1 member A
NCBI Official Synonym Full Names
phospholipase A1 member A
NCBI Official Symbol
Pla1a
NCBI Official Synonym Symbols
Pspla1; Ps-pla1
NCBI Protein Information
phospholipase A1 member A
UniProt Protein Name
Phospholipase A1 member A
Protein Family
UniProt Gene Name
Pla1a
UniProt Synonym Gene Names
Pspla1; PS-PLA1
UniProt Entry Name
PLA1A_RAT
NCBI Description
serine specific phospholipase that catalyzes the hydrolysis of a fatty acyl residue at the sn-1 position of lysophosphatidylserine and phosphatidylserine [RGD, Feb 2006]
Uniprot Description
Function: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1-acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production.2 PublicationsManual assertion based on experiment in:Ref.1
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Product Notes
The Rat PLA1A pla1a (Catalog #AAA9712113) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712113 ELISA Kit recognizes Rat PLA1A. It is sometimes possible for the material contained within the vial of "Phospholipase A1 Member A (PLA1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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