Rabbit anti-Human, Mouse PLA1A Polyclonal Antibody | anti-PLA1A antibody

PLA1A, ID (PLA1A, NMD, PSPLA1, Phospholipase A1 member A, Phosphatidylserine-specific phospholipase A1) (APC)

Cat. Num. AAA6334499

• Gene Names: PLA1A; PSPLA1; PS-PLA1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLA1A; Polyclonal Antibody; ID (PLA1A; NMD; PSPLA1; Phospholipase A1 member A; Phosphatidylserine-specific phospholipase A1) (APC); Phosphatidylserine-specific phospholipase A1; anti-PLA1A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-PLA1A antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: PLA1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336~366 amino acids from the Central region of human PLA1A.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PLA1A Antibody (Center) western blot analysis in mouse liver tissue and CEM cell line lysates (35ug/lane).This demonstrates the PLA1A antibody detected the PLA1A protein (arrow).)

Related Product Information for anti-PLA1A antibody

Phosphatidylserine-specific phospholipase A1-alpha (PLA1A) acts specifically on phosphatidylserine (PS) and 1-acyl-2-lysophosphatidylserine (lyso-PS) to hydrolyze fatty acids at the sn-1 position of these phospholipids.

Product Categories/Family for anti-PLA1A antibody

Antibodies; Enzymes; Phospholipase

References

Morikawa, R.K., et al. J. Biol. Chem. 284(39):26620-26630(2009). Aoki, J., et al. Biochim. Biophys. Acta 1582 (1-3), 26-32 (2002). Wang, J., et al. J. Hum. Genet. 47(11):611-613(2002). Nagai, Y., et al. J. Biol. Chem. 274(16):11053-11059(1999). Van Groningen, J.J., et al. FEBS Lett. 404(1):82-86(1997).

NCBI and Uniprot Product Information

• NCBI GI #: 332688256
• NCBI GeneID: 51365
• NCBI Accession #: NP_001193889.1
• NCBI GenBank Nucleotide #: NM_001206960.1
• UniProt Accession #: Q53H76; O95991; Q86WX6; Q9UPD2; B2R8V2; B4DXA2
• Molecular Weight: 49,715 Da
• NCBI Official Full Name: phospholipase A1 member A isoform 2
• NCBI Official Synonym Full Names: phospholipase A1 member A
• NCBI Official Symbol: PLA1A
• NCBI Official Synonym Symbols: PSPLA1; PS-PLA1
• NCBI Protein Information: phospholipase A1 member A; phosphatidylserine-specific phospholipase A1alpha
• UniProt Protein Name: Phospholipase A1 member A
• Protein Family: Phospholipase
• UniProt Gene Name: PLA1A
• UniProt Synonym Gene Names: NMD; PSPLA1; PS-PLA1
• UniProt Entry Name: PLA1A_HUMAN

NCBI Description

The protein encoded by this gene is a phospholipase that hydrolyzes fatty acids at the sn-1 position of phosphatidylserine and 1-acyl-2-lysophosphatidylserine. This secreted protein hydrolyzes phosphatidylserine in liposomes. Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]

Uniprot Description

PLA1A: Hydrolyzes the ester bond at the sn-1 position of glycerophospholipids and produces 2-acyl lysophospholipids. Hydrolyzes phosphatidylserine (PS) in the form of liposomes and 1- acyl-2 lysophosphatidylserine (lyso-PS), but not triolein, phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidic acid (PA) or phosphatidylinositol (PI). Isoform 2 hydrolyzes lyso-PS but not PS. Hydrolysis of lyso-PS in peritoneal mast cells activated by receptors for IgE leads to stimulate histamine production. Belongs to the AB hydrolase superfamily. Lipase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Phospholipase; EC 3.1.1.-; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q13.13-q13.2

Cellular Component: acrosomal membrane

Molecular Function: phospholipase A1 activity

Biological Process: phosphatidylserine metabolic process; lipid metabolic process; lipid catabolic process