Parathyroid Hormone-Like Related Protein (PTHLH) ELISA Kit | PTHLH elisa kit

Rat Parathyroid Hormone-Like Related Protein (PTHLH) ELISA Kit

Cat. Num. AAA9712022

• Gene Names: PTHLH; PTHRP; PTH-rP
• Reactivity: Rat
• Synonyms: Parathyroid Hormone-Like Related Protein (PTHLH); Rat Parathyroid Hormone-Like Related Protein (PTHLH) ELISA Kit; PTHLH; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel; PTHLH elisa kit

• Reactivity: Rat
• Specificity: Rat Parathyroid Hormone-Like Related Protein (PTHLH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PTHLH. No significant cross-reactivity or interference between Rat PTHLH and analogues was observed.
• Sequence Length: 177

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Parathyroid Hormone-Like Related Protein (PTHLH) Standard Curve.)

Related Product Information for PTHLH elisa kit

Description: This Rat Parathyroid Hormone-Like Related Protein (PTHLH) ELISA Kit employs a two-site sandwich ELISA to quantitate PTHLH in samples. An antibody specific for PTHLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTHLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTHLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTHLH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Parathyroid hormone-related protein (or PTHrP) is a protein occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous cell carcinoma). However, it also has normal functions. PTHrP acts as an endocrine, autocrine/ paracrine, and intracrine hormone. PTHrP is related in function to the "normal" parathyroid hormone. When a tumor secretes PTHrP, this can lead to hypercalcemia. As this is sometimes the first sign of the malignancy, hypercalcemia caused by PTHrP is considered a paraneoplastic phenomenon. PTHrP shares the same N-terminal end as parathyroid hormone and therefore it can bind to the same receptor (the Type I PTH receptor). PTHrP aids in normal mammary gland development and lactation as well as placental transfer of calcium.

NCBI and Uniprot Product Information

• NCBI GI #: 130495876
• NCBI GeneID: 100009357
• NCBI Accession #: NP_001076122.1
• NCBI GenBank Nucleotide #: NP_001076122.1
• UniProt Accession #: Q9GLC7
• NCBI Official Full Name: parathyroid hormone-related protein
• NCBI Official Symbol: PTHLH
• NCBI Official Synonym Symbols: PTHRP; PTH-rP
• NCBI Protein Information: parathyroid hormone-related protein
• UniProt Protein Name: Parathyroid hormone-related protein
• Protein Family: Parathyroid hormone-related protein
• UniProt Gene Name: PTHLH
• UniProt Synonym Gene Names: PTHRP; PTH-rP; PTHrP

Uniprot Description

Neuroendocrine peptide which is a critical regulator of cellular and organ growth, development, migration, differentiation and survival and of epithelial calcium ion transport. Regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. Required for skeletal homeostasis. Promotes mammary mesenchyme differentiation and bud outgrowth by modulating mesenchymal cell responsiveness to BMPs. Upregulates BMPR1A expression in the mammary mesenchyme and this increases the sensitivity of these cells to BMPs and allows them to respond to BMP4 in a paracrine and/or autocrine fashion. BMP4 signaling in the mesenchyme, in turn, triggers epithelial outgrowth and augments MSX2 expression, which causes the mammary mesenchyme to inhibit hair follicle formation within the nipple sheath ().