Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Osteoprotegerin (OPG) Standard Curve.)
Rat Osteoprotegerin (OPG) ELISA Kit | OPG elisa kit
Rat Osteoprotegerin (OPG) ELISA Kit
Gene Names
Tnfrsf11b; Opg
Reactivity
Rat
Synonyms
Osteoprotegerin (OPG); Rat Osteoprotegerin (OPG) ELISA Kit; TNFRSF11B; MGC29565; OCIF; OPG; TR1; osteoclastogenesis inhibitory factor; osteoprotegerin; OPG elisa kit
Reactivity
Rat
Specificity
Rat Osteoprotegerin (OPG) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFRSF11B. No significant cross-reactivity or interference between Rat TNFRSF11B and analogues was observed.
Sequence Length
401
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Osteoprotegerin (OPG) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Osteoprotegerin (OPG) Standard Curve.)
Related Product Information for OPG elisa kit
Description: This Rat Osteoprotegerin (OPG) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF11B in samples. An antibody specific for TNFRSF11B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNFRSF11B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF11B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF11B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Osteoprotegerin (OPG) is a cytokine, which can inhibit the production of osteoclasts. It is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a basic glycoprotein comprising 401 amino acid residues arranged into 7 structural domains. It is found as either a 60 kDa monomer or 120 kDa dimer linked by disulfide bonds.Osteoprotegerin inhibits the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitro and in vivo. Osteoprotegerin is a RANK homolog, and works by binding to RANKL on osteoblast/stromal cells, thus blocking the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
Background: Osteoprotegerin (OPG) is a cytokine, which can inhibit the production of osteoclasts. It is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a basic glycoprotein comprising 401 amino acid residues arranged into 7 structural domains. It is found as either a 60 kDa monomer or 120 kDa dimer linked by disulfide bonds.Osteoprotegerin inhibits the differentiation of osteoclast precursors into osteoclasts and also regulates the resorption of osteoclasts in vitro and in vivo. Osteoprotegerin is a RANK homolog, and works by binding to RANKL on osteoblast/stromal cells, thus blocking the RANKL-RANK ligand interaction between osteoblast/stromal cells and osteoclast precursors. This has the effect of inhibiting the differentiation of the osteoclast precursor into a mature osteoclast.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
tumor necrosis factor receptor superfamily member 11B
NCBI Official Synonym Full Names
TNF receptor superfamily member 11B
NCBI Official Symbol
Tnfrsf11b
NCBI Official Synonym Symbols
Opg
NCBI Protein Information
tumor necrosis factor receptor superfamily member 11B
UniProt Protein Name
Tumor necrosis factor receptor superfamily member 11B
UniProt Gene Name
Tnfrsf11b
UniProt Synonym Gene Names
Opg
NCBI Description
plays a role in the negative regulation of bone resorption by inhibiting formation of osteoclasts [RGD, Feb 2006]
Uniprot Description
Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis ().
Research Articles on OPG
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Product Notes
The Rat OPG tnfrsf11b (Catalog #AAA9711675) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711675 ELISA Kit recognizes Rat OPG. It is sometimes possible for the material contained within the vial of "Osteoprotegerin (OPG), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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