Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Oncostatin M (OSM) Standard Curve.)
Rat Oncostatin M (OSM) ELISA Kit | OSM elisa kit
Rat Oncostatin M (OSM) ELISA Kit
Reactivity
Rat
Synonyms
Oncostatin M (OSM); Rat Oncostatin M (OSM) ELISA Kit; OSM; MGC20461; OSM elisa kit
Reactivity
Rat
Specificity
Rat Oncostatin M (OSM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat OSM. No significant cross-reactivity or interference between Rat OSM and analogues was observed.
Sequence Length
239
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Oncostatin M (OSM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Oncostatin M (OSM) Standard Curve.)
Related Product Information for OSM elisa kit
Description: This Rat Oncostatin M (OSM) ELISA Kit employs a two-site sandwich ELISA to quantitate OSM in samples. An antibody specific for OSM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OSM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Oncostatin M(OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors.
Background: Oncostatin M(OSM) is a cytokine originally isolated from medium conditioned by PMA-treated U-937 histiocytic leukemia cells based on its ability to inhibit growth of A375 melanoma cells. The OSM cDNA encodes a 252 amino acid pre-pro-OSM polypeptide with a 25 residue hydrophobic signal peptide and a hydrophilic C-terminal domain that are proteolytically processed to generate the 196 residue mature form of OSM. Although both mature and pro-OSM are equally active in radio-receptor assays, the mature OSM is 5- to 60-fold more active in growth inhibition assays. Thus, proteolytic processing of the pro-OSM peptide may be important in regulating the in vivo activities of OSM.OSM is a pleiotropic cytokine that initiates its biological activities by binding to specific cell surface receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
oncostatin-M
NCBI Official Synonym Full Names
oncostatin M
NCBI Official Symbol
Osm
NCBI Protein Information
oncostatin-M
UniProt Protein Name
Oncostatin-M
Protein Family
UniProt Gene Name
Osm
UniProt Synonym Gene Names
OSM
UniProt Entry Name
ONCM_RAT
NCBI Description
mouse homolog is a cytokine and an immediate early gene [RGD, Feb 2006]
Uniprot Description
Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells (). Uses only type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration.
Research Articles on OSM
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Product Notes
The Rat OSM osm (Catalog #AAA9712700) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712700 ELISA Kit recognizes Rat OSM. It is sometimes possible for the material contained within the vial of "Oncostatin M (OSM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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