Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Odontogenic ameloblast-associated protein (ODAM) Standard Curve.)
Rat Odontogenic Ameloblast-Associated Protein (ODAM) ELISA Kit | ODAM elisa kit
Rat Odontogenic Ameloblast-Associated Protein (ODAM) ELISA Kit
Gene Names
Odam; Apin
Reactivity
Rat
Synonyms
Odontogenic Ameloblast-Associated Protein (ODAM); Rat Odontogenic Ameloblast-Associated Protein (ODAM) ELISA Kit; ODAM; APIN; FLJ20513; odontogenic ameloblast-associated protein; ODAM elisa kit
Reactivity
Rat
Specificity
Rat Odontogenic ameloblast-associated protein (ODAM) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ODAM. No significant cross-reactivity or interference between Rat ODAM and analogues was observed.
Sequence Length
278
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Odontogenic ameloblast-associated protein (ODAM) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Odontogenic ameloblast-associated protein (ODAM) Standard Curve.)
Related Product Information for ODAM elisa kit
Description: This Rat Odontogenic ameloblast-associated protein (ODAM) ELISA Kit employs a two-site sandwich ELISA to quantitate ODAM in samples. An antibody specific for ODAM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ODAM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ODAM is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ODAM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: ODAM is expressed in rodent enamel organ and is likely involved in dental development.Notably, these reagents immunostained normal and malignant ameloblasts and other types of human neoplastic cells, including those of gastric, lung, and breast origin where the presence in the latter was confirmed by in situ hybridization using gene-specific molecular probes. Moreover, significant titers of anti-ODAM IgG antibodies were detected in the sera of patients with these malignancies.ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms; ODAM may serve as a novel prognostic biomarker, as well as a potential therapeutic target for patients with breast and other epithelial forms of cancer
Background: ODAM is expressed in rodent enamel organ and is likely involved in dental development.Notably, these reagents immunostained normal and malignant ameloblasts and other types of human neoplastic cells, including those of gastric, lung, and breast origin where the presence in the latter was confirmed by in situ hybridization using gene-specific molecular probes. Moreover, significant titers of anti-ODAM IgG antibodies were detected in the sera of patients with these malignancies.ODAM is a developmental antigen that has an essential role in tooth maturation and in the pathogenesis of certain odontogenic and other epithelial neoplasms; ODAM may serve as a novel prognostic biomarker, as well as a potential therapeutic target for patients with breast and other epithelial forms of cancer
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
odontogenic ameloblast-associated protein
NCBI Official Synonym Full Names
odontogenic, ameloblast associated
NCBI Official Symbol
Odam
NCBI Official Synonym Symbols
Apin
NCBI Protein Information
odontogenic ameloblast-associated protein
UniProt Protein Name
Odontogenic ameloblast-associated protein
Protein Family
UniProt Gene Name
Odam
UniProt Synonym Gene Names
Apin
Uniprot Description
Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.
Research Articles on ODAM
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Product Notes
The Rat ODAM odam (Catalog #AAA9712560) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712560 ELISA Kit recognizes Rat ODAM. It is sometimes possible for the material contained within the vial of "Odontogenic Ameloblast-Associated Protein (ODAM), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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