Rabbit anti-Human MRII Polyclonal Antibody | anti-MRII antibody

MRII, CT (Anti-Muellerian Hormone Type II Receptor, Anti-Muellerian Hormone Type II Receptor, AMH Type II Receptor, MIS Type II Receptor, MISRII, AMHR2, AMHR) (MaxLight 490)

Cat. Num. AAA6488285

• Gene Names: AMHR2; AMHR; MRII; MISR2; MISRII
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: MRII; Polyclonal Antibody; CT (Anti-Muellerian Hormone Type II Receptor; Anti-Muellerian Hormone Type II Receptor; AMH Type II Receptor; MIS Type II Receptor; MISRII; AMHR2; AMHR) (MaxLight 490); anti-MRII antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human AMHR2.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-MRII antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human AMHR2 (KLH).
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of AMHR2 Antibody (C-term) in CEM cell line lysates (35ug/lane).AMHR2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using AMHR2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(Western blot analysis of AMHR2 Antibody (C-term) in CEM cell line lysates (35ug/lane).AMHR2 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of CEM cells using AMHR2 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-MRII antibody

Antibodies; Receptors

References

1. Picard, J.Y., et al., J. Soc. Biol. 196(3):217-221 (2002). 2. Teixeira, J., et al., Endocr. Rev. 22(5):657-674 (2001). 3. Imbeaud, S., et al., Nat. Genet. 11(4):382-388 (1995). 4. Visser, J.A., et al., Biochem. Biophys. Res. Commun. 215(3):1029-1036 (1995). 5. Sinisi, A.A., et al., J. Endocrinol. Invest. 26(3 Suppl), 23-28 (2003).

NCBI and Uniprot Product Information

• NCBI GI #: 10198656
• NCBI GeneID: 269
• NCBI Accession #: NP_065434
• NCBI GenBank Nucleotide #: NM_020547.3
• UniProt Accession #: Q16671
• Molecular Weight: 63kDa
• NCBI Official Full Name: anti-Muellerian hormone type-2 receptor isoform 1
• NCBI Official Synonym Full Names: anti-Mullerian hormone receptor type 2
• NCBI Official Symbol: AMHR2
• NCBI Official Synonym Symbols: AMHR; MRII; MISR2; MISRII
• NCBI Protein Information: anti-Muellerian hormone type-2 receptor
• UniProt Protein Name: Anti-Muellerian hormone type-2 receptor
• UniProt Gene Name: AMHR2
• UniProt Synonym Gene Names: AMHR; MISR2; AMH type II receptor; MISRII; MRII
• UniProt Entry Name: AMHR2_HUMAN

NCBI Description

This gene encodes the receptor for the anti-Mullerian hormone (AMH) which, in addition to testosterone, results in male sex differentiation. AMH and testosterone are produced in the testes by different cells and have different effects. Testosterone promotes the development of male genitalia while the binding of AMH to the encoded receptor prevents the development of the mullerian ducts into uterus and Fallopian tubes. Mutations in this gene are associated with persistent Mullerian duct syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2009]

Uniprot Description

MISR2: On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for anti-Muellerian hormone. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.

Protein type: EC 2.7.11.30; Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (receptor); Membrane protein, integral; TKL group; STKR family; Type2 subfamily

Chromosomal Location of Human Ortholog: 12q13

Molecular Function: hormone binding; protein binding; receptor activity

Biological Process: sex differentiation; signal transduction

Disease: Persistent Mullerian Duct Syndrome, Types I And Ii