Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Nicotinate-nucleotide pyrophosphorylase (QPRT) Standard Curve.)
Rat Nicotinate-Nucleotide Pyrophosphorylase (QPRT) ELISA Kit | QPRT elisa kit
Rat Nicotinate-Nucleotide Pyrophosphorylase (QPRT) ELISA Kit
Reactivity
Rat
Synonyms
Nicotinate-Nucleotide Pyrophosphorylase (QPRT); Rat Nicotinate-Nucleotide Pyrophosphorylase (QPRT) ELISA Kit; QPRTase; nicotinate-nucleotide pyrophosphorylase (carboxylating); QPRT elisa kit
Reactivity
Rat
Specificity
Rat Nicotinate-nucleotide pyrophosphorylase (QPRT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat QPRT. No significant cross-reactivity or interference between Rat QPRT and analogues was observed.
Sequence Length
299
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Nicotinate-nucleotide pyrophosphorylase (QPRT) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Nicotinate-nucleotide pyrophosphorylase (QPRT) Standard Curve.)
Related Product Information for QPRT elisa kit
Description: This Rat Nicotinate-nucleotide pyrophosphorylase (QPRT) ELISA Kit employs a two-site sandwich ELISA to quantitate QPRT in samples. An antibody specific for QPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any QPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for QPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of QPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Quinolinate is an intermediate in the de novo synthesis pathway of NAD from tryptophan and acts as a potent endogenous excitotoxin through hyperstimulation of N-methyl D-aspartate (NMDA) receptor in the neuron. Elevation of quinolinate levels in the human brain has been postulated to be involved in the pathogenesis of neurodegenerative disorders. Mammalian quinolinate phosphoribosyltransferase (QPRTase) is a key enzyme in catabolism of quinolinate.This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.
Background: Quinolinate is an intermediate in the de novo synthesis pathway of NAD from tryptophan and acts as a potent endogenous excitotoxin through hyperstimulation of N-methyl D-aspartate (NMDA) receptor in the neuron. Elevation of quinolinate levels in the human brain has been postulated to be involved in the pathogenesis of neurodegenerative disorders. Mammalian quinolinate phosphoribosyltransferase (QPRTase) is a key enzyme in catabolism of quinolinate.This gene encodes a key enzyme in catabolism of quinolinate, an intermediate in the tryptophan-nicotinamide adenine dinucleotide pathway. Quinolinate acts as a most potent endogenous exitotoxin to neurons. Elevation of quinolinate levels in the brain has been linked to the pathogenesis of neurodegenerative disorders such as epilepsy, Alzheimer's disease, and Huntington's disease.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
nicotinate-nucleotide pyrophosphorylase
NCBI Official Synonym Full Names
quinolinate phosphoribosyltransferase
NCBI Official Symbol
Qprt
NCBI Protein Information
nicotinate-nucleotide pyrophosphorylase [carboxylating]
UniProt Protein Name
Nicotinate-nucleotide pyrophosphorylase [carboxylating]
Protein Family
UniProt Gene Name
Qprt
UniProt Synonym Gene Names
QAPRTase; QPRTase
UniProt Entry Name
NADC_RAT
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Product Notes
The Rat QPRT qprt (Catalog #AAA9711996) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9711996 ELISA Kit recognizes Rat QPRT. It is sometimes possible for the material contained within the vial of "Nicotinate-Nucleotide Pyrophosphorylase (QPRT), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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