Rat Neuroglobin (NGB) ELISA Kit | NGB elisa kit

Rat Neuroglobin (NGB) ELISA Kit

Cat. Num. AAA2702144

• Reactivity: Rat
• Synonyms: Neuroglobin (NGB); Rat Neuroglobin (NGB) ELISA Kit; NGB elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Neuroglobin (NGB).; No significant cross-reactivity or interference between Neuroglobin (NGB) and analogues was observed.
• Sequence Length: 151

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.067ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroglobin (NGB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroglobin (NGB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NGB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuroglobin (NGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuroglobin (NGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuroglobin (NGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuroglobin (NGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10864065
• NCBI GeneID: 58157
• NCBI Accession #: NP_067080.1
• NCBI GenBank Nucleotide #: NM_021257.3
• UniProt Accession #: Q9NPG2
• Molecular Weight: 16,933 Da
• NCBI Official Full Name: neuroglobin
• NCBI Official Synonym Full Names: neuroglobin
• NCBI Official Symbol: NGB
• NCBI Protein Information: neuroglobin
• UniProt Protein Name: Neuroglobin
• Protein Family: Neuroglobin
• UniProt Gene Name: NGB

NCBI Description

This gene encodes an oxygen-binding protein that is distantly related to members of the globin gene family. It is highly conserved among other vertebrates. It is expressed in the central and peripheral nervous system where it may be involved in increasing oxygen availability and providing protection under hypoxic/ischemic conditions. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.