Rat Myosin Light Chain 2 (MYL2) ELISA Kit | MYL2 elisa kit

Rat Myosin Light Chain 2 (MYL2) ELISA Kit

Cat. Num. AAA2707521

• Gene Names: MYL2; MLC2; CMH10; MLC-2s/v
• Reactivity: Rat
• Synonyms: Myosin Light Chain 2 (MYL2); Rat Myosin Light Chain 2 (MYL2) ELISA Kit; CMH10; MLC2; Myosin;Light Chain 2;Regulatory;Cardiac;Slow; Myosin regulatory light chain 2; ventricular/cardiac muscle isoform; Myosin Light Chain 2; Regulatory; Cardiac; MYL2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 2 (MYL2).; No significant cross-reactivity or interference between Myosin Light Chain 2 (MYL2) and analogues was observed.
• Sequence Length: 166

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 29pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 2 (MYL2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 2 (MYL2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MYL2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2 (MYL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 2 (MYL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 2 (MYL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 2 (MYL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94981553
• NCBI GeneID: 4633
• NCBI GenBank Nucleotide #: NP_000423.2
• UniProt Accession #: P10916
• NCBI Official Full Name: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• NCBI Official Synonym Full Names: myosin light chain 2
• NCBI Official Symbol: MYL2
• NCBI Official Synonym Symbols: MLC2; CMH10; MLC-2s/v
• NCBI Protein Information: myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• UniProt Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform
• Protein Family: Myosin regulatory
• UniProt Gene Name: MYL2
• UniProt Synonym Gene Names: MLC-2; MLC-2v
• UniProt Entry Name: MLRV_HUMAN

NCBI Description

Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008]

Uniprot Description

MRLC2V: myosin regulatory light chain 2, ventricular/cardiac muscle isoform. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy.

Protein type: Contractile

Chromosomal Location of Human Ortholog: 12q24.11

Cellular Component: sarcomere; cytoskeleton; myofibril; myosin complex; cytosol; A band; actin cytoskeleton

Molecular Function: actin monomer binding; protein binding; structural constituent of muscle; calcium ion binding; myosin heavy chain binding

Biological Process: muscle cell fate specification; heart contraction; regulation of striated muscle contraction; ventricular cardiac muscle morphogenesis; cardiac myofibril assembly; negative regulation of cell growth; muscle fiber development; post-embryonic development; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Familial Hypertrophic, 10